Molecular sexing of tigers, Panthera tigris

• Main Authors: McEwing, R., Ouitavon, K., Rovie-Ryan, J. J., M., Wulansari, Sitam, F. T., Ogden, R.
• Format: Article
• Language: English
• Published: Springer Nature 2011
• Subjects: QL Zoology
• Online Access: http://ir.unimas.my/id/eprint/36007/1/tigris1.pdf; http://ir.unimas.my/id/eprint/36007/; https://link.springer.com/article/10.1007/s12686-011-9529-x; https://doi.org/10.1007/s12686-011-9529-x
• Institution: Universiti Malaysia Sarawak

We report the development of a fast and reliable PCR-based method for sex identification of tiger DNA designed to be incorporated into fluorescent short tandem repeat (STR) profiling. A single primer pair, consisting of a fluorescently-labelled forward primer and an unlabelled reverse primer, is used to co-amplify homologous fragments of a zinc finger (ZF) protein intron which exhibits size polymorphism between the X and Y chromosomes. The ZFX and ZFY amplicons differ in size by 12 bp and can thus be differentiated by capillary electrophoresis.