Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase I (COI) and D-loop region sequences

Mousedeer is one of the primitive mammals that can be found mainly in Southeast-Asia region. There are two species of mousedeer in Malaysia which are Tragulus kanchil and Tragulus napu. Both species can be distinguish by size, coat coloration, and throat pattern but clear diagnosis still cannot be f...

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Main Authors: Mohamad Azam Akmal, Abu Bakar, Rovie Ryan, Jeffrine Japning, Ahmad, Ampeng, Salmah, Yaakop, Shukor, Md Nor, Badrul Munir, Md Zain
Format: Proceeding
Language:English
Published: 2018
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Online Access:http://ir.unimas.my/id/eprint/36066/1/jeffrine.pdf
http://ir.unimas.my/id/eprint/36066/
https://doi.org/10.1063/1.5027950
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Institution: Universiti Malaysia Sarawak
Language: English
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spelling my.unimas.ir.360662023-03-29T03:16:36Z http://ir.unimas.my/id/eprint/36066/ Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase I (COI) and D-loop region sequences Mohamad Azam Akmal, Abu Bakar Rovie Ryan, Jeffrine Japning Ahmad, Ampeng Salmah, Yaakop Shukor, Md Nor Badrul Munir, Md Zain QL Zoology Mousedeer is one of the primitive mammals that can be found mainly in Southeast-Asia region. There are two species of mousedeer in Malaysia which are Tragulus kanchil and Tragulus napu. Both species can be distinguish by size, coat coloration, and throat pattern but clear diagnosis still cannot be found. The objective of the study is to show the genetic distance relationship between T. kanchil and T. napu and their population based on mitochondrial DNA (mtDNA) cytochrome oxidase I (COI) and D-loop region. There are 42 sample of mousedeer were used in this study collected by PERHILITAN from different locality. Another 29 D-loop sequence were retrieved from Genbank for comparative analysis. All sample were amplified using universal primer and species-specific primer for COI and D-loop genes via PCR process. The amplified sequences were analyzed to determine genetic distance of T. kanchil and T. napu. From the analysis, the average genetic distance between T. kanchil and T. napu based on locus COI and D-loop were 0.145 and 0.128 respectively. The genetic distance between populations of T. kanchil based on locus COI was between 0.003-0.013. For locus D-loop, genetic distance analysis showed distance in relationship between west-coast populations to east-coast population of T. kanchil. COI and D-loop mtDNA region provided a clear picture on the relationship within the mousedeer species. Last but not least, conservation effort toward protecting this species can be done by study the molecular genetics and prevent the extinction of this species. 2018 Proceeding PeerReviewed text en http://ir.unimas.my/id/eprint/36066/1/jeffrine.pdf Mohamad Azam Akmal, Abu Bakar and Rovie Ryan, Jeffrine Japning and Ahmad, Ampeng and Salmah, Yaakop and Shukor, Md Nor and Badrul Munir, Md Zain (2018) Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase I (COI) and D-loop region sequences. In: The 2017 UKM FST Postgraduate Colloquium, 12-13 July 2017, Selangor, Malaysia. https://doi.org/10.1063/1.5027950 doi:10.1063/1.5027950
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QL Zoology
spellingShingle QL Zoology
Mohamad Azam Akmal, Abu Bakar
Rovie Ryan, Jeffrine Japning
Ahmad, Ampeng
Salmah, Yaakop
Shukor, Md Nor
Badrul Munir, Md Zain
Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase I (COI) and D-loop region sequences
description Mousedeer is one of the primitive mammals that can be found mainly in Southeast-Asia region. There are two species of mousedeer in Malaysia which are Tragulus kanchil and Tragulus napu. Both species can be distinguish by size, coat coloration, and throat pattern but clear diagnosis still cannot be found. The objective of the study is to show the genetic distance relationship between T. kanchil and T. napu and their population based on mitochondrial DNA (mtDNA) cytochrome oxidase I (COI) and D-loop region. There are 42 sample of mousedeer were used in this study collected by PERHILITAN from different locality. Another 29 D-loop sequence were retrieved from Genbank for comparative analysis. All sample were amplified using universal primer and species-specific primer for COI and D-loop genes via PCR process. The amplified sequences were analyzed to determine genetic distance of T. kanchil and T. napu. From the analysis, the average genetic distance between T. kanchil and T. napu based on locus COI and D-loop were 0.145 and 0.128 respectively. The genetic distance between populations of T. kanchil based on locus COI was between 0.003-0.013. For locus D-loop, genetic distance analysis showed distance in relationship between west-coast populations to east-coast population of T. kanchil. COI and D-loop mtDNA region provided a clear picture on the relationship within the mousedeer species. Last but not least, conservation effort toward protecting this species can be done by study the molecular genetics and prevent the extinction of this species.
format Proceeding
author Mohamad Azam Akmal, Abu Bakar
Rovie Ryan, Jeffrine Japning
Ahmad, Ampeng
Salmah, Yaakop
Shukor, Md Nor
Badrul Munir, Md Zain
author_facet Mohamad Azam Akmal, Abu Bakar
Rovie Ryan, Jeffrine Japning
Ahmad, Ampeng
Salmah, Yaakop
Shukor, Md Nor
Badrul Munir, Md Zain
author_sort Mohamad Azam Akmal, Abu Bakar
title Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase I (COI) and D-loop region sequences
title_short Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase I (COI) and D-loop region sequences
title_full Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase I (COI) and D-loop region sequences
title_fullStr Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase I (COI) and D-loop region sequences
title_full_unstemmed Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase I (COI) and D-loop region sequences
title_sort genetic distance of malaysian mousedeer based on mitochondrial dna cytochrome oxidase i (coi) and d-loop region sequences
publishDate 2018
url http://ir.unimas.my/id/eprint/36066/1/jeffrine.pdf
http://ir.unimas.my/id/eprint/36066/
https://doi.org/10.1063/1.5027950
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