A Novel Technique Using Advanced Oxidation Process (UV-C/H2O2) Combined with Micro-Nano Bubbles on Decontamination, Seed Viability, and Enhancing Phytonutrients of Roselle Microgreens
Microbial contamination commonly occurs in microgreens due to contaminated seeds. This study investigated the decontamination effects of water wash (control), 5% hydrogen peroxide (H2O2 ), UV-C (36 watts), advanced oxidation process (AOP; H2O2 + UV-C), and improved AOP by combination with microb...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI
2022
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/38220/1/A%20Novel%20Technique%20Using%20Advanced%20Oxidation%20Process.pdf http://ir.unimas.my/id/eprint/38220/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Universiti Malaysia Sarawak |
| Language: | English |
| Summary: | Microbial contamination commonly occurs in microgreens due to contaminated seeds.
This study investigated the decontamination effects of water wash (control), 5% hydrogen peroxide
(H2O2
), UV-C (36 watts), advanced oxidation process (AOP; H2O2 + UV-C), and improved AOP by
combination with microbubbles (MBs; H2O2 + MBs and H2O2 + UV-C + MBs) on microbial loads,
seeds’ viability, and physio-biochemical properties of microgreens from corresponding roselle seeds.
Results showed that H2O2 and AOP, with and without MBs, significantly reduced total aerobic
bacteria, coliforms, Escherichia coli (E. coli), and molds and yeast log count in seeds as compared to
the control. Improved AOP treatment of H2O2 + UV-C + MBs significantly augmented antimicrobial
activity against total bacteria and E. coli (not detected,) as compared to control and other treatments
due to the formation of the highest hydroxy radicals (5.25 × 10−13 M). Additionally, H2O2 and
combined treatments promoted seed germination, improved microbiological quality, total phenolic,
flavonoids, and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•
) activity of the grown microgreens.
Ascorbic acid content was induced only in microgreens developed from H2O2
-treated seeds. Single
UV-C treatment was ineffective to inactivate the detected microorganism population in seeds. These
findings demonstrated that improved AOP treatment (H2O2 + UV-C + MBs) could potentially be
used as a new disinfection technology for seed treatment in microgreens production. |
|---|