Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa)
A total of 35 Kanagawa-negative strains of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) were investigated by randomly amplified polymorphic DNA fingerprinting with three primers and their plasmid profiles. Eighteen strains carried small plasmid(s) of 2.4 to 7.3kb that enabled the...
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Rapid Science Publishers
1998
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Online Access: | http://ir.unimas.my/id/eprint/39192/1/Use%20of%20randomly%20-%20Copy.pdf http://ir.unimas.my/id/eprint/39192/ https://link.springer.com/article/10.1023/A:1008841514368 |
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my.unimas.ir.391922022-08-15T00:28:23Z http://ir.unimas.my/id/eprint/39192/ Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) Son, Radu Nasreldin, E. H. Zaiton, H. Samuel, Lihan Rusul, G. Nimita, F. Q Science (General) A total of 35 Kanagawa-negative strains of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) were investigated by randomly amplified polymorphic DNA fingerprinting with three primers and their plasmid profiles. Eighteen strains carried small plasmid(s) of 2.4 to 7.3kb that enabled the V. parahaemolyticus to be grouped into eight plasmid patterns. The three primers generated polymorphisms in all 35 strains of V. parahaemolyticus tested, producing bands ranging from 0.25 to 3.9kb. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested, and that cockles in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. Rapid Science Publishers 1998 Article PeerReviewed text en http://ir.unimas.my/id/eprint/39192/1/Use%20of%20randomly%20-%20Copy.pdf Son, Radu and Nasreldin, E. H. and Zaiton, H. and Samuel, Lihan and Rusul, G. and Nimita, F. (1998) Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa). World Journal of Microbiology and Biotechnology, 14. pp. 895-901. ISSN 1573-0972 https://link.springer.com/article/10.1023/A:1008841514368 DOI https://doi.org/10.1023/A:1008841514368 |
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Q Science (General) Son, Radu Nasreldin, E. H. Zaiton, H. Samuel, Lihan Rusul, G. Nimita, F. Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) |
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A total of 35 Kanagawa-negative strains of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) were investigated by randomly amplified polymorphic DNA fingerprinting with three primers and their plasmid profiles. Eighteen strains carried small plasmid(s) of 2.4 to 7.3kb that enabled the V. parahaemolyticus to be grouped into eight plasmid patterns. The three primers generated polymorphisms in all 35 strains of V. parahaemolyticus tested, producing bands ranging from 0.25 to 3.9kb. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested, and that cockles in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. |
format |
Article |
author |
Son, Radu Nasreldin, E. H. Zaiton, H. Samuel, Lihan Rusul, G. Nimita, F. |
author_facet |
Son, Radu Nasreldin, E. H. Zaiton, H. Samuel, Lihan Rusul, G. Nimita, F. |
author_sort |
Son, Radu |
title |
Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) |
title_short |
Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) |
title_full |
Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) |
title_fullStr |
Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) |
title_full_unstemmed |
Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) |
title_sort |
use of randomly amplified polymorphic dna analysis to differentiate isolates of vibrio parahaemolyticus from cockles (anadara granosa) |
publisher |
Rapid Science Publishers |
publishDate |
1998 |
url |
http://ir.unimas.my/id/eprint/39192/1/Use%20of%20randomly%20-%20Copy.pdf http://ir.unimas.my/id/eprint/39192/ https://link.springer.com/article/10.1023/A:1008841514368 |
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1743110937358893056 |