EXPERIMENTAL DEPRESSION-MODULATED ACTIVATION PATTERN OF THE HYPOTHALAMIC-PITUITARY-ADRENAL AXIS

Objective: To study the effect of modelled depression not associated with stress on the hypothalamic-pituitary-adrenal system (HPAS) in mice. Methods: The experiment was conducted on the twenty-four C57BL/6 mice weighing 20-25 g, divided into two groups: an experimental one, involving animals with...

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Main Authors: Nur Amirah Diyana, Razlan, Marina, Kapitonova, Renad Nikolaevich, Alyautdin, Saiful Bahri, Talip, Norhida, Ramli, Tin Moe, Nwe
Format: Article
Language:English
Published: Avicenna Tajik State Medical University & National Academy of Sciences of Tajikistan 2024
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Online Access:http://ir.unimas.my/id/eprint/44590/3/EXPERIMENTAL.pdf
http://ir.unimas.my/id/eprint/44590/
https://vestnik-avicenna.tj/en/archive/2024/2024-1/experimental-depression-modulated-activation-pattern-of-the-hypothalamic-pituitary-adrenal-axis/?sphrase_id=172663
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spelling my.unimas.ir.445902024-04-18T01:43:21Z http://ir.unimas.my/id/eprint/44590/ EXPERIMENTAL DEPRESSION-MODULATED ACTIVATION PATTERN OF THE HYPOTHALAMIC-PITUITARY-ADRENAL AXIS Nur Amirah Diyana, Razlan Marina, Kapitonova Renad Nikolaevich, Alyautdin Saiful Bahri, Talip Norhida, Ramli Tin Moe, Nwe R Medicine (General) Objective: To study the effect of modelled depression not associated with stress on the hypothalamic-pituitary-adrenal system (HPAS) in mice. Methods: The experiment was conducted on the twenty-four C57BL/6 mice weighing 20-25 g, divided into two groups: an experimental one, involving animals with reserpine-induced depression, and a control one, the animals of which were administered normal saline instead of reserpine solution. At the end of the experiment, behavioral tests were performed (open field, sucrose preference, forced swimming), and the corticosterone concentration in blood was measured using ELISA. Image analysis of histological changes in the adrenal gland and thymus was performed. All results were statistically processed using SPSS 27.0.1 software, with p<0.05 indicating the significance of differences. Results: Animals of the experimental group showed a significantly higher level of corticosterone in the blood (p<0.01), significant changes in behavior in the open field test (distance traveled, rearing, frequency of urination and defecation, p<0.01), reduced sucrose preference (p<0.01) and shortened duration of the active phase in the forced swimming test (p<0.01) than the animals of the control group. Besides, in the mice of the experimental group, a significant increase in the volume density of the zona fasciculata of the adrenal cortex was found (p<0.05), as well as the area of its spongiocytes and their nuclei (p<0.05). In the thymus of the experimental animals, a significant decrease in the cortex-medulla ratio was noted (p<0.05); and an increase in the volume density of tingible body macrophages (p<0.05) compared to the control group of mice. Conclusion: Modelled depression, not associated with stress, the depth of which was assessed by behavioral tests, caused activation of the HPAS, manifested by increased corticosterone levels, hyperplasia of the zona fasciculata of the adrenal cortex, hypertrophy of its spongiocytes and their nuclei, as well as changes in the thymus, indicating associated immunosuppression. Thus, the study showed that, along with the well-known phenomenon of the development of depression against the background of chronic stress-associated activation of HPAS, there is an inverse mechanism leading to activation of the HPAS during the development of depression not associated with stress, which must be taken into account when developing the new strategies of treating depression. Avicenna Tajik State Medical University & National Academy of Sciences of Tajikistan 2024 Article PeerReviewed text en http://ir.unimas.my/id/eprint/44590/3/EXPERIMENTAL.pdf Nur Amirah Diyana, Razlan and Marina, Kapitonova and Renad Nikolaevich, Alyautdin and Saiful Bahri, Talip and Norhida, Ramli and Tin Moe, Nwe (2024) EXPERIMENTAL DEPRESSION-MODULATED ACTIVATION PATTERN OF THE HYPOTHALAMIC-PITUITARY-ADRENAL AXIS. Avicenna Bulletin, 26 (1). pp. 57-66. ISSN 2959-6327 https://vestnik-avicenna.tj/en/archive/2024/2024-1/experimental-depression-modulated-activation-pattern-of-the-hypothalamic-pituitary-adrenal-axis/?sphrase_id=172663 doi: 10.25005/2074-0581-2024-26-1-57-66
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Nur Amirah Diyana, Razlan
Marina, Kapitonova
Renad Nikolaevich, Alyautdin
Saiful Bahri, Talip
Norhida, Ramli
Tin Moe, Nwe
EXPERIMENTAL DEPRESSION-MODULATED ACTIVATION PATTERN OF THE HYPOTHALAMIC-PITUITARY-ADRENAL AXIS
description Objective: To study the effect of modelled depression not associated with stress on the hypothalamic-pituitary-adrenal system (HPAS) in mice. Methods: The experiment was conducted on the twenty-four C57BL/6 mice weighing 20-25 g, divided into two groups: an experimental one, involving animals with reserpine-induced depression, and a control one, the animals of which were administered normal saline instead of reserpine solution. At the end of the experiment, behavioral tests were performed (open field, sucrose preference, forced swimming), and the corticosterone concentration in blood was measured using ELISA. Image analysis of histological changes in the adrenal gland and thymus was performed. All results were statistically processed using SPSS 27.0.1 software, with p<0.05 indicating the significance of differences. Results: Animals of the experimental group showed a significantly higher level of corticosterone in the blood (p<0.01), significant changes in behavior in the open field test (distance traveled, rearing, frequency of urination and defecation, p<0.01), reduced sucrose preference (p<0.01) and shortened duration of the active phase in the forced swimming test (p<0.01) than the animals of the control group. Besides, in the mice of the experimental group, a significant increase in the volume density of the zona fasciculata of the adrenal cortex was found (p<0.05), as well as the area of its spongiocytes and their nuclei (p<0.05). In the thymus of the experimental animals, a significant decrease in the cortex-medulla ratio was noted (p<0.05); and an increase in the volume density of tingible body macrophages (p<0.05) compared to the control group of mice. Conclusion: Modelled depression, not associated with stress, the depth of which was assessed by behavioral tests, caused activation of the HPAS, manifested by increased corticosterone levels, hyperplasia of the zona fasciculata of the adrenal cortex, hypertrophy of its spongiocytes and their nuclei, as well as changes in the thymus, indicating associated immunosuppression. Thus, the study showed that, along with the well-known phenomenon of the development of depression against the background of chronic stress-associated activation of HPAS, there is an inverse mechanism leading to activation of the HPAS during the development of depression not associated with stress, which must be taken into account when developing the new strategies of treating depression.
format Article
author Nur Amirah Diyana, Razlan
Marina, Kapitonova
Renad Nikolaevich, Alyautdin
Saiful Bahri, Talip
Norhida, Ramli
Tin Moe, Nwe
author_facet Nur Amirah Diyana, Razlan
Marina, Kapitonova
Renad Nikolaevich, Alyautdin
Saiful Bahri, Talip
Norhida, Ramli
Tin Moe, Nwe
author_sort Nur Amirah Diyana, Razlan
title EXPERIMENTAL DEPRESSION-MODULATED ACTIVATION PATTERN OF THE HYPOTHALAMIC-PITUITARY-ADRENAL AXIS
title_short EXPERIMENTAL DEPRESSION-MODULATED ACTIVATION PATTERN OF THE HYPOTHALAMIC-PITUITARY-ADRENAL AXIS
title_full EXPERIMENTAL DEPRESSION-MODULATED ACTIVATION PATTERN OF THE HYPOTHALAMIC-PITUITARY-ADRENAL AXIS
title_fullStr EXPERIMENTAL DEPRESSION-MODULATED ACTIVATION PATTERN OF THE HYPOTHALAMIC-PITUITARY-ADRENAL AXIS
title_full_unstemmed EXPERIMENTAL DEPRESSION-MODULATED ACTIVATION PATTERN OF THE HYPOTHALAMIC-PITUITARY-ADRENAL AXIS
title_sort experimental depression-modulated activation pattern of the hypothalamic-pituitary-adrenal axis
publisher Avicenna Tajik State Medical University & National Academy of Sciences of Tajikistan
publishDate 2024
url http://ir.unimas.my/id/eprint/44590/3/EXPERIMENTAL.pdf
http://ir.unimas.my/id/eprint/44590/
https://vestnik-avicenna.tj/en/archive/2024/2024-1/experimental-depression-modulated-activation-pattern-of-the-hypothalamic-pituitary-adrenal-axis/?sphrase_id=172663
_version_ 1797543491914432512