Polymorphism in the tumor necrosis factor alpha promoter region and its influence on colorectal cancer predisposition risk in Malaysian population
objective:A case control study was designed to investigate the TNF-a -308 G>A polymorphism allele frequencies and to determine the influence of the polymorphic genotype on sporadic CRC susceptibility risk in Malaysia population. Material and methods: Peripheral blood samples of 164 normal contro...
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| Main Authors: | , , , , , , , , |
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| Format: | E-Article |
| Language: | English |
| Published: |
Japan International Cultural Exchange Foundation (JICEF),
2011
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/706/1/Polymorphism%20in%20the%20tumor%20necrosis%20factor%20alpha%20promoter.pdf http://ir.unimas.my/id/eprint/706/ http://www.seronjihou.co.jp/imj/ |
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| Institution: | Universiti Malaysia Sarawak |
| Language: | English |
| Summary: | objective:A case control study was designed to investigate the TNF-a -308 G>A polymorphism allele frequencies and to determine the influence of the polymorphic genotype on sporadic CRC susceptibility risk in Malaysia population.
Material and methods: Peripheral blood samples of 164 normal controls and 161 clinically and histopathhologically confirm CRC patients were genotyped for TNF-a -308 G>A polymorphism employing allele specific PCR. The relative associations of various genotypes with CRC susceptibility risk was determined by calculating Odd ratios. Corresponding Chi-square tests on CRC patients and controls were carried out and 95% confidence interval (95% CI) were determined using fisher exact tests.
Results: On comparing the frequencies of genotypes of patients and controls, the homozygous variant AA was significabtly higher in CRC patients (p=0.030) compared to controls. On investigating the association of the polymorphic genotypes with CRC susceptibility risk, the homozygous variant TNF-a -308AA showed significantly increased risk with OR 2.5842.
Conclusion: Our results suggest that, polymorphic genotype of inflammation response gene TNF-a is significantly associated with CRC susceptibility risk and colud be considered as a high risk variant for CRC predisposition. |
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