Isolation and Characterization of Carbazole and Dibenzofuran Degrading Bacteria from Soil in Mangrove Environment
Heterocyclic hydrocarbons are toxic and mutagenic components of petroleum and creosote contamination that are frequently found in the groundwater, seawater, sediments, and soil sites. A study was carried out to isolate and characterize heterocyclic hydrocarbon degrading bacteria from soil samples...
Saved in:
| Main Author: | |
|---|---|
| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2013
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/7540/1/Fatin%20Nur%20Sufinas%20ft.pdf http://ir.unimas.my/id/eprint/7540/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Universiti Malaysia Sarawak |
| Language: | English |
| Summary: | Heterocyclic hydrocarbons are toxic and mutagenic components of petroleum and creosote contamination that
are frequently found in the groundwater, seawater, sediments, and soil sites. A study was carried out to isolate
and characterize heterocyclic hydrocarbon degrading bacteria from soil samples collected at mangrove
environment at Asajaya, Sarawak. This environment has physicochemical characteristics of high salinity, tidal
range, strong winds, high temperature, crude oil and muddy soil. Crude oil often contains various heterocyclic
compounds that are toxic for most life forms. Despite its toxicity, some bacteria species have the capability to
mineralize these heterocyclic hydrocarbons compound efficiently. Two bacteria strains FNS01 and FNS02 was
isolated and grown with enrichment of carbazole (CAR) and dibenzofuran (DBF) as the sole source of carbon.
Characterization of these two isolated bacteria include motility test with the use of semi-solid medium, catalase
test, salt tolerance test, hydrogen sulfide test, oxidase test, gram staining, and also different polycyclic aromatic
hydrocarbons (PAHs) substrate test such as carbazole (CAR), biphenyl (BPH), fluorene (FLO), dibenzofuran
(DBF), and dibenzothiophene (DBT) were studied. Total DNA was extracted and 16S rRNA amplified by PCR
method and subsequently differentiates the species with restriction fragment length polymorphism (RFLP).
Isolation of these strains might be useful in the bioremediation of environments contaminated by heterocyclic hydrocarbon. |
|---|