Transformation of isochorismate synthase in fungal system
Agrobacterium-mediated transformation is a powerful tool that allows the introduction of particular genetic changes in an organism. Transformation of fungi, Aspergillus niger is necessary to fulfill the increasing global industrial demand. Isochorismate synthase gene from Morinda citrifolia is im...
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| Main Author: | |
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| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2013
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/7625/1/Lim%20Pei%20Wen%20ft.pdf http://ir.unimas.my/id/eprint/7625/ |
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| Institution: | Universiti Malaysia Sarawak |
| Language: | English |
| Summary: | Agrobacterium-mediated transformation is a powerful tool that allows the introduction of
particular genetic changes in an organism. Transformation of fungi, Aspergillus niger is
necessary to fulfill the increasing global industrial demand. Isochorismate synthase gene
from Morinda citrifolia is important in the involvement of salicylic acid which plays a role
in plant immunological system. In this study, the isolated plasmids pGSA1131/ICS were
successfully transformed into Agrobacterium tumefaciens cells. A. tumefaciens carrying
pGSA1131/ICS containing bacterial BAR-resistant gene have been used in A. niger
transformation. Different concentration of growth media containing different concentration
of selective agent, Basta, were used in the transformation to obtain a maximum yield of
transformants. The growth of mycelia on top agar of PDA plates containing reduced
concentration of Basta at 10 mg/L. The extracted fungal DNA was examined via PCR. A
successful transformation of A. niger was achieved. |
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