Characterization of marine penicillium isolates and their antibiotics

• Main Author: Mohd.Noorzaifiqrudin, Khairol
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2012
• Subjects: QR Microbiology
• Online Access: http://ir.unimas.my/id/eprint/8124/3/Noorzaifiqrudin%20full.pdf; http://ir.unimas.my/id/eprint/8124/
• Institution: Universiti Malaysia Sarawak

brought to human and animal health. Penicillium sp. have been known as their potential of producing antimicrobial compounds. Recently, this species were discovered to produce more than one type of antimicrobial compounds. This discovery has been promising to encounter the ongoing emergence of antibiotic resistant microorganisms. In this study, a total of five pure fungal isolates were cultivated from marine environment. Out of five, two of them were putatively identified as P. oxalicum and Ampelomyces sp. From the preliminary test of fungi isolates, a'imost all the isolates were shown to have strong antibacterial activity against test bacteria, which were S. aureus, a Gram-positive bacteria as well as S. typhi, E. coli and E. aerogenes, Gramnegative bacteria. Contrary to this, only dichloromethane extracts of isolates SVM3 and SVP4 (PDA) were active against S. aureus observed in disc diffusion assay. The results were also inconsistent with the bioautography assay of the extracts, which showed only dichloromethane extracts of SVP4 (CDA) and SVM3 were active against S. aureus and E. aerogenes, respectively. The active compound in bioautography assay might be penicillin, as compared to penicillin-streptomycin as reference.