Identification of genetic lesions in the ribosomal protein gene, RPL41 from human nasopharyngeal derived cell lines

Nasopharyngeal carcinoma (NPC) is associated with three RP genes, namely RPL27, RPL37a and RPL41 that were significantly downregulated in all cell lines derived from NPC tissues when compared with nonmalignant nasopharyngeal epithelial cell lines. However, there is no report on RPL41 genes in human...

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Main Author:	Sultana Parvin, Binti Habeebur Rahman
Format:	Final Year Project Report
Language:	English
Published:	Universiti Malaysia Sarawak, (UNIMAS) 2012
Subjects:	Q Science (General) QM Human anatomy
Online Access:	http://ir.unimas.my/id/eprint/8208/4/Sultana%20%28fulltext%29.pdf http://ir.unimas.my/id/eprint/8208/
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Institution:	Universiti Malaysia Sarawak
Language:	English

id	my.unimas.ir.8208
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spelling	my.unimas.ir.82082024-02-08T03:31:31Z http://ir.unimas.my/id/eprint/8208/ Identification of genetic lesions in the ribosomal protein gene, RPL41 from human nasopharyngeal derived cell lines Sultana Parvin, Binti Habeebur Rahman Q Science (General) QM Human anatomy Nasopharyngeal carcinoma (NPC) is associated with three RP genes, namely RPL27, RPL37a and RPL41 that were significantly downregulated in all cell lines derived from NPC tissues when compared with nonmalignant nasopharyngeal epithelial cell lines. However, there is no report on RPL41 genes in human nasopharyngeal tissue. The objective of this study is to investigate the correlation between NPC and RPL41 gene sequence using nom1al and tumour cell lines derived from nasopharyngeal tissues. Total RNA of cell lines were used as the starting material and were transcribed into cDNA using M-MLV transcriptase. The 227 bp RPL41 gene was successfully amplified by polymerase chain reaction and was subsequently purified and sent for sequencing. The result shows that there is no nucleotide variation been observed in the coding sequences of RPL41 among these cell lines. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/8208/4/Sultana%20%28fulltext%29.pdf Sultana Parvin, Binti Habeebur Rahman (2012) Identification of genetic lesions in the ribosomal protein gene, RPL41 from human nasopharyngeal derived cell lines. [Final Year Project Report] (Unpublished)
institution	Universiti Malaysia Sarawak
building	Centre for Academic Information Services (CAIS)
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sarawak
content_source	UNIMAS Institutional Repository
url_provider	http://ir.unimas.my/
language	English
topic	Q Science (General) QM Human anatomy
spellingShingle	Q Science (General) QM Human anatomy Sultana Parvin, Binti Habeebur Rahman Identification of genetic lesions in the ribosomal protein gene, RPL41 from human nasopharyngeal derived cell lines
description	Nasopharyngeal carcinoma (NPC) is associated with three RP genes, namely RPL27, RPL37a and RPL41 that were significantly downregulated in all cell lines derived from NPC tissues when compared with nonmalignant nasopharyngeal epithelial cell lines. However, there is no report on RPL41 genes in human nasopharyngeal tissue. The objective of this study is to investigate the correlation between NPC and RPL41 gene sequence using nom1al and tumour cell lines derived from nasopharyngeal tissues. Total RNA of cell lines were used as the starting material and were transcribed into cDNA using M-MLV transcriptase. The 227 bp RPL41 gene was successfully amplified by polymerase chain reaction and was subsequently purified and sent for sequencing. The result shows that there is no nucleotide variation been observed in the coding sequences of RPL41 among these cell lines.
format	Final Year Project Report
author	Sultana Parvin, Binti Habeebur Rahman
author_facet	Sultana Parvin, Binti Habeebur Rahman
author_sort	Sultana Parvin, Binti Habeebur Rahman
title	Identification of genetic lesions in the ribosomal protein gene, RPL41 from human nasopharyngeal derived cell lines
title_short	Identification of genetic lesions in the ribosomal protein gene, RPL41 from human nasopharyngeal derived cell lines
title_full	Identification of genetic lesions in the ribosomal protein gene, RPL41 from human nasopharyngeal derived cell lines
title_fullStr	Identification of genetic lesions in the ribosomal protein gene, RPL41 from human nasopharyngeal derived cell lines
title_full_unstemmed	Identification of genetic lesions in the ribosomal protein gene, RPL41 from human nasopharyngeal derived cell lines
title_sort	identification of genetic lesions in the ribosomal protein gene, rpl41 from human nasopharyngeal derived cell lines
publisher	Universiti Malaysia Sarawak, (UNIMAS)
publishDate	2012
url	http://ir.unimas.my/id/eprint/8208/4/Sultana%20%28fulltext%29.pdf http://ir.unimas.my/id/eprint/8208/
_version_	1792160577985970176

Identification of genetic lesions in the ribosomal protein gene, RPL41 from human nasopharyngeal derived cell lines

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