Antiviral activity of Cymbopogon nardus methanol fractions against measles disease virus
Measles disease virus has been a major concern since it has not been able to be fully eradicated. Vaccine was introduced and surveillances were conducted for the disease. The disease still claims the life of thousands of children especially in developing countries where vaccine and drugs are not...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2014
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| Online Access: | http://ir.unimas.my/id/eprint/8791/1/Reagan%20Entigu%20Linton%20ft.pdf http://ir.unimas.my/id/eprint/8791/ |
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| Institution: | Universiti Malaysia Sarawak |
| Language: | English |
| Summary: | Measles disease virus has been a major concern since it has not been able to be fully
eradicated. Vaccine was introduced and surveillances were conducted for the disease. The
disease still claims the life of thousands of children especially in developing countries where
vaccine and drugs are not easily accessible to the people. Active constituents from medicinal
plant products were studied and their bioactivities were identified. The chemical structure
allows researchers to better understand the mechanisms of infection, hence enable mass
production of the compound either naturally or synthetically activity-enhanced compound.
Methanol fractions and subfractions of Cymbopogon nardus, serai wangi, were tested for
antiviral activity against measles virus and their cytotoxicity against ovarian cancer cell line,
Caov3 compared to Vero cell line were evaluated. The assays were carried out based on
bioassay-guided fractionation and the antiviral mechanism was evaluated. The fraction C2
showed the highest activity at post-infection assay and its subfraction C2.1 was the most
active. The subfraction was run on thin-layer chromatography plate to separate the
constituents, yielding four individual spots where the second spot, C2.1b has the active
antiviral and highly cytotoxic toward Caov3 cell line. The GC/MS analysis revealed that it
contained octadecanoic acid, methyl ester, a fatty acid. The findings showed that the methanol
fraction of C. nardus possessed potential antiviral activity and selective toxicity against
ovarian cancer cell. Further study on active compounds of the C. nardus against different
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viruses, human cell lines and also other bioactivities such as antimicrobial should be carried
out. |
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