Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002

• Main Authors: Muhammad Suhaib, M. H., Mohd Hasnain, Hussain, Awang Ahmad Sallehin, Awang Husaini, Kopli, Bujang, Dayang Salwani, Awang Adeni, Mohd Azib, Salleh
• Format: Article
• Language: English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2013
• Subjects: Q Science (General); QR Microbiology
• Online Access: http://ir.unimas.my/id/eprint/9829/1/Preliminary.pdf; http://ir.unimas.my/id/eprint/9829/; http://www.researchgate.net/publication/280813911
• Institution: Universiti Malaysia Sarawak

Characterization of α-amylase gene sequence produced by Bacillus amyloliquefaciens UMAS 1002, a cellulolytic and amylolytic bacilli isolated from sago pith waste is described here. The amyE gene encoding the α-amylase was isolated by polymerase chain reaction. The 1,980 bp of amyE gene corresponding to 660 amino acids showed 99% homology to the α-amylase sequence from Bacillus subtilis X-23 (GenBank: BAA31528). The α-amylase sequence of B. amyloliquefaciens UMAS 1002 (GenBank: KC800929) differs from that of B. subtilis X-23 by 5 amino acids. In silico analysis of α-amylase from B. amyloliquefaciens UMAS 1002 showed similar characteristics compared to α-amylase from B. subtilis X-23