Chemical Composition, Antioxidant, Antibacterial, and Antibiofilm Activities of Backhousia citriodora Essential Oil

antiinfective agent; antioxidant; essential oil; biofilm; chemistry; Escherichia coli; microbial sensitivity test; Myrtaceae; Staphylococcus aureus; Staphylococcus epidermidis; Anti-Bacterial Agents; Antioxidants; Biofilms; Escherichia coli; Microbial Sensitivity Tests; Myrtaceae; Oils, Volatile; St...

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Main Authors:	Lim A.C., Tang S.G.H., Zin N.M., Maisarah A.M., Ariffin I.A., Ker P.J., Mahlia T.M.I.
Other Authors:	57853430200
Format:	Article
Published:	MDPI 2023
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Institution:	Universiti Tenaga Nasional

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spelling	my.uniten.dspace-268052023-05-29T17:36:51Z Chemical Composition, Antioxidant, Antibacterial, and Antibiofilm Activities of Backhousia citriodora Essential Oil Lim A.C. Tang S.G.H. Zin N.M. Maisarah A.M. Ariffin I.A. Ker P.J. Mahlia T.M.I. 57853430200 57853430300 18039045200 57196273927 57189225370 37461740800 56997615100 antiinfective agent; antioxidant; essential oil; biofilm; chemistry; Escherichia coli; microbial sensitivity test; Myrtaceae; Staphylococcus aureus; Staphylococcus epidermidis; Anti-Bacterial Agents; Antioxidants; Biofilms; Escherichia coli; Microbial Sensitivity Tests; Myrtaceae; Oils, Volatile; Staphylococcus aureus; Staphylococcus epidermidis The essential oil of Backhousia citriodora, commonly known as lemon myrtle oil, possesses various beneficial properties due to its richness in bioactive compounds. This study aimed to characterize the chemical profile of the essential oil isolated from leaves of Backhousia citriodora (BCEO) and its biological properties, including antioxidant, antibacterial, and antibiofilm activities. Using gas chromatography�mass spectrometry, 21 compounds were identified in BCEO, representing 98.50% of the total oil content. The isomers of citral, geranial (52.13%), and neral (37.65%) were detected as the main constituents. The evaluation of DPPH radical scavenging activity and ferric reducing antioxidant power showed that BCEO exhibited strong antioxidant activity at IC50 of 42.57 ?g/mL and EC50 of 20.03 ?g/mL, respectively. The antibacterial activity results showed that BCEO exhibited stronger antibacterial activity against Gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis) than against Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae). For the agar disk diffusion method, S. epidermidis was the most sensitive to BCEO with an inhibition zone diameter of 50.17 mm, followed by S. aureus (31.13 mm), E. coli (20.33 mm), and K. pneumoniae (12.67 mm). The results from the microdilution method showed that BCEO exhibited the highest activity against S. epidermidis and S. aureus, with the minimal inhibitory concentration (MIC) value of 6.25 ?L/mL. BCEO acts as a potent antibiofilm agent with dual actions, inhibiting (85.10% to 96.44%) and eradicating (70.92% to 90.73%) of the biofilms formed by the four tested bacteria strains, compared with streptomycin (biofilm inhibition, 67.65% to 94.29% and biofilm eradication, 49.97% to 89.73%). This study highlights that BCEO can potentially be a natural antioxidant agent, antibacterial agent, and antibiofilm agent that could be applied in the pharmaceutical and food industries. To the best of the authors� knowledge, this is the first report, on the antibiofilm activity of BCEO against four common nosocomial pathogens. � 2022 by the authors. Final 2023-05-29T09:36:50Z 2023-05-29T09:36:50Z 2022 Article 10.3390/molecules27154895 2-s2.0-85136340172 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85136340172&doi=10.3390%2fmolecules27154895&partnerID=40&md5=8b529542bda46641e86ac4af1cf33a9e https://irepository.uniten.edu.my/handle/123456789/26805 27 15 4895 All Open Access, Gold, Green MDPI Scopus
institution	Universiti Tenaga Nasional
building	UNITEN Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Tenaga Nasional
content_source	UNITEN Institutional Repository
url_provider	http://dspace.uniten.edu.my/
description	antiinfective agent; antioxidant; essential oil; biofilm; chemistry; Escherichia coli; microbial sensitivity test; Myrtaceae; Staphylococcus aureus; Staphylococcus epidermidis; Anti-Bacterial Agents; Antioxidants; Biofilms; Escherichia coli; Microbial Sensitivity Tests; Myrtaceae; Oils, Volatile; Staphylococcus aureus; Staphylococcus epidermidis
author2	57853430200
author_facet	57853430200 Lim A.C. Tang S.G.H. Zin N.M. Maisarah A.M. Ariffin I.A. Ker P.J. Mahlia T.M.I.
format	Article
author	Lim A.C. Tang S.G.H. Zin N.M. Maisarah A.M. Ariffin I.A. Ker P.J. Mahlia T.M.I.
spellingShingle	Lim A.C. Tang S.G.H. Zin N.M. Maisarah A.M. Ariffin I.A. Ker P.J. Mahlia T.M.I. Chemical Composition, Antioxidant, Antibacterial, and Antibiofilm Activities of Backhousia citriodora Essential Oil
author_sort	Lim A.C.
title	Chemical Composition, Antioxidant, Antibacterial, and Antibiofilm Activities of Backhousia citriodora Essential Oil
title_short	Chemical Composition, Antioxidant, Antibacterial, and Antibiofilm Activities of Backhousia citriodora Essential Oil
title_full	Chemical Composition, Antioxidant, Antibacterial, and Antibiofilm Activities of Backhousia citriodora Essential Oil
title_fullStr	Chemical Composition, Antioxidant, Antibacterial, and Antibiofilm Activities of Backhousia citriodora Essential Oil
title_full_unstemmed	Chemical Composition, Antioxidant, Antibacterial, and Antibiofilm Activities of Backhousia citriodora Essential Oil
title_sort	chemical composition, antioxidant, antibacterial, and antibiofilm activities of backhousia citriodora essential oil
publisher	MDPI
publishDate	2023
_version_	1806428132898504704

Chemical Composition, Antioxidant, Antibacterial, and Antibiofilm Activities of Backhousia citriodora Essential Oil

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