Dengue envelope domain III-peptide binding analysis via tryptophan fluorescence quenching assay

• Main Authors: Baharuddin, A., Hassan, A.A., Othman, R., Xu, Y., Huang, M., Ario Tejo, B., Yusof, R., Rahman, N.A., Othman, S.
• Format: Article
• Language: English
• Published: 2017
• Institution: Universiti Tenaga Nasional

In the efforts to find an anti-viral treatment for dengue, a simple tryptophan fluorescence-screening assay aimed at identifying dengue domain III envelope (EIII) protein inhibitors was developed. Residue Trp391 of EIII was used as an intrinsic probe to monitor the change in fluorescence of the tryp...