DNA free CRISPR/DCAS9 based transcriptional activation system for UGT76G1 gene in Stevia rebaudiana Bertoni protoplasts
The UDP-glycosyltransferase 76G1 (UGT76G1) is responsible for the conversion of stevioside to rebaudioside A. Four single guide RNAs (sgRNAs) were designed from the UGT76G1 proximal promoter region of stevia by using the online-based tool, benchling. The dCas9 fused with VP64 as a transcriptional ac...
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| Main Authors: | , , , |
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| Format: | Article |
| Published: |
MDPI
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/100989/ https://www.mdpi.com/2223-7747/11/18/2393 |
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| Institution: | Universiti Putra Malaysia |