Improving of pelB-Secreted MPT64 protein released by Escherichia coli BL21 (DE3) using Triton X-100 and Tween-80

• Main Authors: Kusuma, Sri Agung Fitri, Subroto, Toto, Parwati, Ida, Rukayadi, Yaya, Fadhlillah, Muhammad, Pardede, Ruth Michellee
• Format: Article
• Published: Wolters Kluwer Medknow Publications 2022
• Online Access: http://psasir.upm.edu.my/id/eprint/101825/; https://journals.lww.com/japtr/toc/2022/13030
• Institution: Universiti Putra Malaysia

pelB has been known as a successful signal peptide to translocate the protein target extracellularly in the Escherichia coli system. However, in our previous study, the yield of MPT64 protein extracellular recovery was still low and plenty of this protein was remain trapped in cytoplasm and periplas...