Investigation of serum and milk acute phase proteins level in goats with experimentally infected staphylococcus aureus subclinical mastitis

Mastitis is an inflammatory condition of the mammary gland which is usually caused by a bacterial infection. This study aims to determine acute phase protein (APP) and its association with somatic cell count (SCC) during experimentally induced subclinical mastitis in goats. Thirty lactating goats we...

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Main Authors: Tajol Ariffin, Muhammad F, Chai, M Hian, Sukiman, Muhammad Z, Ghazali, Mohd F, Zainal Ariffin, Siti M
Format: Article
Published: ResearchersLinks 2022
Online Access:http://psasir.upm.edu.my/id/eprint/101992/
https://researcherslinks.com/current-issues/Investigation-of-Serum-and-Milk-Acute-Phase-Proteins-Level-in-Goats-with-Experimentally-Infected-Staphylococcus-aureus-Subclinical-Mastitis/34/1/4844/html
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Institution: Universiti Putra Malaysia
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spelling my.upm.eprints.1019922024-03-15T04:21:08Z http://psasir.upm.edu.my/id/eprint/101992/ Investigation of serum and milk acute phase proteins level in goats with experimentally infected staphylococcus aureus subclinical mastitis Tajol Ariffin, Muhammad F Chai, M Hian Sukiman, Muhammad Z Ghazali, Mohd F Zainal Ariffin, Siti M Mastitis is an inflammatory condition of the mammary gland which is usually caused by a bacterial infection. This study aims to determine acute phase protein (APP) and its association with somatic cell count (SCC) during experimentally induced subclinical mastitis in goats. Thirty lactating goats were divided into two groups (n=15 per group) challenged either by intramammary infusion of 1 x 103 cfu/mL Staphylococcus aureus (S. aureus) or phosphate-buffered saline (control). The haptoglobin (Hp), serum amyloid A (SAA) and α1-acid glycoprotein (AGP) levels in serum and milk were measured at pre-and post-infection (6, 24, 48 and 72 h) using the ELISA method. The SCC was determined by the direct microscopic method. Results revealed a time-dependent change of Hp, SAA and AGP in the infected group compared to the control. The APP levels in the milk were significantly higher (p<0.05) than those in the serum. A significant good correlation between milk Hp concentration and SCC was observed (rs= 0.73, p<0.05). However, there were no associations between milk SAA and AGP concentrations with SCC respectively. In conclusion, altered expression of the serum and milk APPs was seen during S. aureus-induced subclinical mastitis infection in goats, which unlock the potentials of APP evaluation as alternative tools for early diagnosis of subclinical mastitis in goats. Also, there was a significant relationship between milk Hp and SCC, thus reflecting the intensity of the inflammatory response to mammary tissue. ResearchersLinks 2022 Article PeerReviewed Tajol Ariffin, Muhammad F and Chai, M Hian and Sukiman, Muhammad Z and Ghazali, Mohd F and Zainal Ariffin, Siti M (2022) Investigation of serum and milk acute phase proteins level in goats with experimentally infected staphylococcus aureus subclinical mastitis. Journal of Animal Health and Production, 10 (1). 121 - 128. ISSN 2308-2801 https://researcherslinks.com/current-issues/Investigation-of-Serum-and-Milk-Acute-Phase-Proteins-Level-in-Goats-with-Experimentally-Infected-Staphylococcus-aureus-Subclinical-Mastitis/34/1/4844/html 10.17582/journal.jahp/2022/10.1.121.128
institution Universiti Putra Malaysia
building UPM Library
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country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Mastitis is an inflammatory condition of the mammary gland which is usually caused by a bacterial infection. This study aims to determine acute phase protein (APP) and its association with somatic cell count (SCC) during experimentally induced subclinical mastitis in goats. Thirty lactating goats were divided into two groups (n=15 per group) challenged either by intramammary infusion of 1 x 103 cfu/mL Staphylococcus aureus (S. aureus) or phosphate-buffered saline (control). The haptoglobin (Hp), serum amyloid A (SAA) and α1-acid glycoprotein (AGP) levels in serum and milk were measured at pre-and post-infection (6, 24, 48 and 72 h) using the ELISA method. The SCC was determined by the direct microscopic method. Results revealed a time-dependent change of Hp, SAA and AGP in the infected group compared to the control. The APP levels in the milk were significantly higher (p<0.05) than those in the serum. A significant good correlation between milk Hp concentration and SCC was observed (rs= 0.73, p<0.05). However, there were no associations between milk SAA and AGP concentrations with SCC respectively. In conclusion, altered expression of the serum and milk APPs was seen during S. aureus-induced subclinical mastitis infection in goats, which unlock the potentials of APP evaluation as alternative tools for early diagnosis of subclinical mastitis in goats. Also, there was a significant relationship between milk Hp and SCC, thus reflecting the intensity of the inflammatory response to mammary tissue.
format Article
author Tajol Ariffin, Muhammad F
Chai, M Hian
Sukiman, Muhammad Z
Ghazali, Mohd F
Zainal Ariffin, Siti M
spellingShingle Tajol Ariffin, Muhammad F
Chai, M Hian
Sukiman, Muhammad Z
Ghazali, Mohd F
Zainal Ariffin, Siti M
Investigation of serum and milk acute phase proteins level in goats with experimentally infected staphylococcus aureus subclinical mastitis
author_facet Tajol Ariffin, Muhammad F
Chai, M Hian
Sukiman, Muhammad Z
Ghazali, Mohd F
Zainal Ariffin, Siti M
author_sort Tajol Ariffin, Muhammad F
title Investigation of serum and milk acute phase proteins level in goats with experimentally infected staphylococcus aureus subclinical mastitis
title_short Investigation of serum and milk acute phase proteins level in goats with experimentally infected staphylococcus aureus subclinical mastitis
title_full Investigation of serum and milk acute phase proteins level in goats with experimentally infected staphylococcus aureus subclinical mastitis
title_fullStr Investigation of serum and milk acute phase proteins level in goats with experimentally infected staphylococcus aureus subclinical mastitis
title_full_unstemmed Investigation of serum and milk acute phase proteins level in goats with experimentally infected staphylococcus aureus subclinical mastitis
title_sort investigation of serum and milk acute phase proteins level in goats with experimentally infected staphylococcus aureus subclinical mastitis
publisher ResearchersLinks
publishDate 2022
url http://psasir.upm.edu.my/id/eprint/101992/
https://researcherslinks.com/current-issues/Investigation-of-Serum-and-Milk-Acute-Phase-Proteins-Level-in-Goats-with-Experimentally-Infected-Staphylococcus-aureus-Subclinical-Mastitis/34/1/4844/html
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