In Vitro Culture and Symbiotic Relationships of Paphiopedilum Niveum, Taeniophyllum Obtusum and Vanda Hookeriana with Their Associated Fungi
In vitro cultures of three orchid species, namely, Paphiopedilum niveum, Taeniophyllum obtusum and Yanda hookeriana, were initiated from different parts of the plants. Proliferous protocorm-like bodies were obtained from shoot tips and axillary buds of V. hookeriana and shoot tips and in flores...
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Main Author: | |
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Format: | Thesis |
Language: | English English |
Published: |
1987
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Subjects: | |
Online Access: | http://psasir.upm.edu.my/id/eprint/10230/1/FP_1987_8.pdf http://psasir.upm.edu.my/id/eprint/10230/ |
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Institution: | Universiti Putra Malaysia |
Language: | English English |
Summary: | In vitro cultures of three orchid species, namely,
Paphiopedilum niveum, Taeniophyllum obtusum and Yanda
hookeriana, were initiated from different parts of the plants.
Proliferous protocorm-like bodies were obtained from shoot
tips and axillary buds of V. hookeriana and shoot tips and
in florescence of T. obtusum. Shoot tips and axillary buds of
P. niveum grew into single or multiple plantlets.
In florescence culture of T. obtusum developed into multiple
branches of in florescences, calli or produced roots. Young
leaf tip or leaf base of P. niveum and root of T. obtusum were
unable to differentiate into callus. However, culture of
young flower bud of P. niveum produced leaf after seven
months. High pH of the medium was suitable for T. obtusum cultures.
Low concentration of modified Knudson's C medium was
preferable to the standard concentration for the culture of
P. niveum. |
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