Optimization of signal peptide via site-directed mutagenesis for enhanced secretion of heterologous proteins in lactococcus lactis

• Main Authors: Alias, Nur Aqlili Riana, Song, Adelene Ai Lian, Alitheen, Noorjahan Banu, Rahim, Raha Abdul, Othman, Siti Sarah, In, Lionel Lian Aun
• Format: Article
• Published: MDPI AG 2022
• Online Access: http://psasir.upm.edu.my/id/eprint/102407/; https://www.mdpi.com/1422-0067/23/17/10044
• Institution: Universiti Putra Malaysia

Secretion efficiency of heterologous proteins in the Generally Regarded As Safe (GRAS) Lactococcus lactis is often reported to be insufficiently low due to limitations such as poor targeting and translocation by the signal peptide or degradation by the host proteases. In this study, the secretion ef...