Reverting TP53 mutation in breast cancer cells: prime editing workflow and technical considerations

• Main Authors: Abuhamad, Asmaa Y., Mohamad Zamberi, Nurul Nadia, Sheen, Ling, Naes, Safaa M., Mohd Yusuf, Siti Nur Hasanah, Ahmad Tajudin, Asilah, Mohtar, M. Aiman, Amir Hamzah, Amir Syahir, Syafruddin, Saiful Effendi
• Format: Article
• Published: MDPI 2022
• Online Access: http://psasir.upm.edu.my/id/eprint/103079/; https://www.mdpi.com/2073-4409/11/10/1612
• Institution: Universiti Putra Malaysia

Breast cancer is the leading cause of cancer-related deaths in women. The aggressive breast cancer subtype is commonly linked to the genetic alterations in the TP53 tumor suppressor gene, predominantly the missense mutations. Robust experimental models are needed to gain better insights into these m...