Altering the regioselectivity of T1 lipase from Geobacillus zalihae toward sn-3 acylglycerol using a rational design approach

• Main Authors: Albayati, Samah Hashim, Masomian, Malihe, Ishak, Siti Nor Hasmah, Leow, Adam Thean Chor, Mohamad Ali, Mohd Shukuri, Mohd Shariff, Fairolniza, Muhd Noor, Noor Dina, Raja Abd Rahman, Raja Noor Zaliha
• Format: Article
• Published: Multidisciplinary Digital Publishing Institute 2023
• Online Access: http://psasir.upm.edu.my/id/eprint/106486/; https://www.mdpi.com/2073-4344/13/2/416
• Institution: Universiti Putra Malaysia

The regioselectivity characteristic of lipases facilitate a wide range of novel molecule unit constructions and fat modifications. Lipases can be categorized as sn-1,3, sn-2, and random regiospecific. Geobacillus zalihae T1 lipase catalyzes the hydrolysis of the sn-1,3 acylglycerol chain. The T1 lip...