Antibacterial and wound healing activity of 2% formulation of 2-Medpy-3-CN on infected burn wounded animal model

• Main Authors: Neela, Vasantha Kumari, Ahmad, Nor Maziah, Wada, Surajo Adamu, Karunanidhi, Arun Kumar
• Format: Article
• Language: English
• Published: Universiti Putra Malaysia 2023
• Online Access: http://psasir.upm.edu.my/id/eprint/106605/1/Antibacterial%20and%20Wound%20Healing.pdf; http://psasir.upm.edu.my/id/eprint/106605/; https://medic.upm.edu.my/upload/dokumen/2023051610430101_MJMHS_1063.pdf
• Institution: Universiti Putra Malaysia

Introduction: Humans have learned to recognize and process plants into medicinal forms through centuries. Burns can spread to other tissues, especially when infected with bacteria such as Methicillin-Resistant Staphylococcus aureus (MRSA). The study aimed to assess the in vivo antibacterial and wound healing activity of 2% formulation of 2-Medpy-3-CN on infected burn wounded animal model. Methods: In vitro antibacterial activity of the Alsti was done by broth dilution and disc diffusion methods. Alsti 2% ointment was prepared for the infected burn wound treatment. A total of 18 rats are grouped into A, B, C, and D, the first three groups (A-C) were injured thermally, and Group D was used as healthy controls. The three test Groups were exposed to MRSA ATCC 43300 at 105 CFU/mL. Group A was treated with 2% Alsti, Group B with Silver sulfadiazine 1% (SSD), and Group C was untreated. Wounds healing was assessed by the healed area and microscopic identification of hematoxylin and eosin (H&E)-stained skin tissue. Results: Wound healing progresses with application of Alsti 2% ointment as observed through wound diameter and histopathological changes of the skin. Wound diameter decreases with treatments, while the contrary was observed in the non-treated group. Microscopic observation of the stained skin showed that epidermal development, and collagen formation progress with treatment days. Untreated wounds showed marked inflammation, progressive ulceration, and necrosis. Conclusion: Alsti 2% formulation showed antibacterial and wound healing activities, hence, can be used as alternative in burn wound infections.