Metabolite alteration associated with Dabai pulp oil supplementation in hypercholesterolemic rats

• Main Authors: Abdul Kadir, Noor Atiqah Aizan, Azlan, Azrina, Abdul Mutalib, Maisarah
• Format: Article
• Language: English
• Published: Universiti Putra Malaysia 2024
• Online Access: http://psasir.upm.edu.my/id/eprint/111522/1/MJMHS_0879.pdf; http://psasir.upm.edu.my/id/eprint/111522/; https://medic.upm.edu.my/upload/dokumen/2024052916594419_MJMHS_0879.pdf
• Institution: Universiti Putra Malaysia

Introduction: Metabolomic analyses have become paramount in unveiling the therapeutic capacities of bioactive agents. Dabai pulp oil (DPO) has emerged as a prospective agent against hypercholesterolemia. This investigation delineates the metabolic imprints of DPO’s therapeutic actions using 1H NMR-based urinary metabolomic profiling. Methods: Male Sprague-Dawley rats were first exposed to a high-cholesterol regimen to simulate hypercholesterolemia. Following this induction, they were transitioned to a DPO-infused diet. The ensuing metabolic variations were tracked using 1H NMR-based urinary metabolomic analysis. Results: The metabolic landscape displayed discernible shifts post-DPO administration, underlining its therapeutic potential. There was a marked decrement in the concentrations of pivotal metabolites such as creatinine, succinate, pyruvate, acetate, TMAO, and choline (p<0.05). Notably, an augmented taurine concentration after DPO administration spotlighted the oil’s antioxidative and anti-inflammatory prowess (p<0.05). These observations underscore DPO’s proficiency in rectifying metabolic aberrations inherent to hypercholesterolemia, particularly affecting energy transduction and cardiovascular function. Conclusion: This empirical evidence bolsters the notion that DPO harbours potent therapeutic virtues for hypercholesterolemia amelioration. Nevertheless, in-depth explorations are quintessential to decoding its holistic therapeutic pathways, fortifying its role in future targeted interventions.