Diploid Gynogenesis in Lampam Jawa Puntius gonionotus using UV irradiated sperm of Puntius schwanenfeldii followed by temperature shock

• Main Authors: Siraj, Siti Shapor, Seki, Shingo, Jee, Ang Kok, Yamada, Yukio, Taniguchi, Nobuhiko
• Format: Article
• Language: English
• Published: The Japanese Society of Fisheries Science 1993
• Online Access: http://psasir.upm.edu.my/id/eprint/111927/1/111927.pdf; http://psasir.upm.edu.my/id/eprint/111927/; https://www.jstage.jst.go.jp/article/suisan1932/59/6/59_6_957/_article
• Institution: Universiti Putra Malaysia

The effects of genetic manipulation of eggs and sperm on the yields of gynogenetic fry of Puntius gonionotus were investigated. Gynogenesis was achieved by cold-and heat-shocking eggs fertilized with ultraviolet irradiated sperm of P. schwanenfeldii at various times after fertilization and at different duration intervals. 66.6% viable, gynogenetic fry were obtained when eggs were inseminated with irradiated sperm and cold shocked at 2°C for 5 minutes duration 1 minute after fertilization. At warm water temperature shocks the fertilized eggs performed best at 42°C, with percent survival rates of 20.0% 1 minute after fertilization for 1.0 minute duration and 17.2% and 14.7% 13 and 23 minutes after fertilization respectively for a duration of 1.5 minutes.