Enzymatic alcoholysis of triolein to produce wax ester

An alcoholysis reaction between triolein and oleyl alcohol catalyzed by Lipozyme and Novozyme was carried out to produce oleyl oleate, a wax ester. The effects of various reaction parameters such as time, reaction temperature, amount of enzyme, molar ratio of substrates (oleyl alcohol/triolein), var...

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Main Authors: Mat Hadzir, Nursyamsyila, Basri, Mahiran, Abd Rahman, Mohd Basyaruddin, Abdul Razak, Che Nyonya, Abd Rahman, Raja Noor Zaliha, Salleh, Abu Bakar
Format: Article
Language:English
Published: Wiley 2001
Online Access:http://psasir.upm.edu.my/id/eprint/112438/3/112438.pdf
http://psasir.upm.edu.my/id/eprint/112438/
https://onlinelibrary.wiley.com/doi/10.1002/jctb.407
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Institution: Universiti Putra Malaysia
Language: English
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spelling my.upm.eprints.1124382025-02-05T01:48:41Z http://psasir.upm.edu.my/id/eprint/112438/ Enzymatic alcoholysis of triolein to produce wax ester Mat Hadzir, Nursyamsyila Basri, Mahiran Abd Rahman, Mohd Basyaruddin Abdul Razak, Che Nyonya Abd Rahman, Raja Noor Zaliha Salleh, Abu Bakar An alcoholysis reaction between triolein and oleyl alcohol catalyzed by Lipozyme and Novozyme was carried out to produce oleyl oleate, a wax ester. The effects of various reaction parameters such as time, reaction temperature, amount of enzyme, molar ratio of substrates (oleyl alcohol/triolein), various organic solvents used and the initial water activity, aw of the reaction system were studied. The best conditions tested to produce wax ester were respectively, incubation time, 5h; temperature, 50°C for Lipozyme and 60°C for Novozyme; weight of enzyme, 0.30g and molar ratio of oleyl alcohol to triolein, 6:1. The use of organic solvents greatly influenced the activity of lipase. Generally, the activity of lipase was high in nonpolar solvents with log P values greater than 2.50. Heptane and hexane were the best solvents tested. The enzymatic synthesis of oleyl was best carried out at aw 0.32. Analysis of the yield of the products of the reaction at optimized reaction condition using Lipozyme showed that 75.66% oleyl oleate was produced. Wiley 2001 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/112438/3/112438.pdf Mat Hadzir, Nursyamsyila and Basri, Mahiran and Abd Rahman, Mohd Basyaruddin and Abdul Razak, Che Nyonya and Abd Rahman, Raja Noor Zaliha and Salleh, Abu Bakar (2001) Enzymatic alcoholysis of triolein to produce wax ester. Journal of Chemical Technology & Biotechnology, 76 (5). pp. 511-515. ISSN 0268-2575; eISSN: 1097-4660 https://onlinelibrary.wiley.com/doi/10.1002/jctb.407 10.1002/jctb.407
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description An alcoholysis reaction between triolein and oleyl alcohol catalyzed by Lipozyme and Novozyme was carried out to produce oleyl oleate, a wax ester. The effects of various reaction parameters such as time, reaction temperature, amount of enzyme, molar ratio of substrates (oleyl alcohol/triolein), various organic solvents used and the initial water activity, aw of the reaction system were studied. The best conditions tested to produce wax ester were respectively, incubation time, 5h; temperature, 50°C for Lipozyme and 60°C for Novozyme; weight of enzyme, 0.30g and molar ratio of oleyl alcohol to triolein, 6:1. The use of organic solvents greatly influenced the activity of lipase. Generally, the activity of lipase was high in nonpolar solvents with log P values greater than 2.50. Heptane and hexane were the best solvents tested. The enzymatic synthesis of oleyl was best carried out at aw 0.32. Analysis of the yield of the products of the reaction at optimized reaction condition using Lipozyme showed that 75.66% oleyl oleate was produced.
format Article
author Mat Hadzir, Nursyamsyila
Basri, Mahiran
Abd Rahman, Mohd Basyaruddin
Abdul Razak, Che Nyonya
Abd Rahman, Raja Noor Zaliha
Salleh, Abu Bakar
spellingShingle Mat Hadzir, Nursyamsyila
Basri, Mahiran
Abd Rahman, Mohd Basyaruddin
Abdul Razak, Che Nyonya
Abd Rahman, Raja Noor Zaliha
Salleh, Abu Bakar
Enzymatic alcoholysis of triolein to produce wax ester
author_facet Mat Hadzir, Nursyamsyila
Basri, Mahiran
Abd Rahman, Mohd Basyaruddin
Abdul Razak, Che Nyonya
Abd Rahman, Raja Noor Zaliha
Salleh, Abu Bakar
author_sort Mat Hadzir, Nursyamsyila
title Enzymatic alcoholysis of triolein to produce wax ester
title_short Enzymatic alcoholysis of triolein to produce wax ester
title_full Enzymatic alcoholysis of triolein to produce wax ester
title_fullStr Enzymatic alcoholysis of triolein to produce wax ester
title_full_unstemmed Enzymatic alcoholysis of triolein to produce wax ester
title_sort enzymatic alcoholysis of triolein to produce wax ester
publisher Wiley
publishDate 2001
url http://psasir.upm.edu.my/id/eprint/112438/3/112438.pdf
http://psasir.upm.edu.my/id/eprint/112438/
https://onlinelibrary.wiley.com/doi/10.1002/jctb.407
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