Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection

• Main Authors: Yahaya, H., Cheah, Y.K., Chee, H.Y., Than, L.T.L.
• Format: Article
• Published: Springer Science and Business Media Deutschland 2024
• Online Access: http://psasir.upm.edu.my/id/eprint/114237/; https://link.springer.com/article/10.1007/s10096-024-04934-4?error=cookies_not_supported&code=4082e6b1-a021-4412-9a8d-95655f03156c
• Institution: Universiti Putra Malaysia

Purpose: Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid method for DNA amplification at a constant temperature. The “gold standard” culture method for yeast detection, has low sensitivity with severe consequences, increasing morbidity and mortality rates. Here, we report the development of a LAMP method for the specific detection of C. glabrata. Methodology: The specific LAMP primers for C. glabrata detection were designed and evaluated. Results: The LAMP assay accurately detected C. glabrata with no cross-reactivity with other Candida species. Conclusion: The developed molecular method would be a promising tool in the management of invasive candidiasis.