EtOH-LN cryoembedding workflow to minimize freezing artifact in frozen tissues: A pilot study in preparing tissues compatible with mass spectrometry-based spatial proteomics application

• Main Authors: Tan, Yong Chiang, Mustangin, Muaatamarulain, Rosli, Nurwahyuna, Wan Ahmad Kammal, Wan Syahira Ellani, Md Isa, Nurismah, Low, Teck Yew, Lee, Pey Yee, Chellappan, Dinesh Kumar, Jarmin, Razman, Zuhdi, Zamri, Azman, Azlanudin, Ian, Chik, Mohamed Yusof, Nursuhadah, Lim, Lay Cheng
• Format: Article
• Language: English
• Published: Academic Press Inc. 2024
• Online Access: http://psasir.upm.edu.my/id/eprint/115064/1/115064.pdf; http://psasir.upm.edu.my/id/eprint/115064/; https://linkinghub.elsevier.com/retrieve/pii/S0011224023003425
• Institution: Universiti Putra Malaysia

Coolant-assisted liquid nitrogen (LN) flash freezing of frozen tissues has been widely adopted to preserve tissue morphology for histopathological annotations in mass spectrometry-based spatial proteomics techniques. However, existing coolants pose health risks upon inhalation and are expensive. To...