Virus Screening Of Cultured Carp And Tilapia Species And The Virus Sensitivity Of Cell Cultures From Indigenous Fish Species
Three virus particles were isolated in vitro and tentatively classified into three groupings , namely Herpesviridae, Birnaviridae and Iridoviridae . Virus groupings were based on the ultrastructural morphology and cytopathology observed . Distinct virus-like cytopathic effects were noted on fou...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
1994
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| Online Access: | http://psasir.upm.edu.my/id/eprint/11797/1/FPSS_1994_4_A.pdf http://psasir.upm.edu.my/id/eprint/11797/ |
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| Institution: | Universiti Putra Malaysia |
| Language: | English English |
| Summary: | Three virus particles were isolated in vitro and
tentatively classified into three groupings , namely
Herpesviridae, Birnaviridae and Iridoviridae . Virus
groupings were based on the ultrastructural morphology and
cytopathology observed . Distinct virus-like cytopathic
effects were noted on four established fish cell lines
viz . CHSE-214 , GCK-84 , EPC and TmB , utilized in the
study . The virus - like particles , from pelleted infected
cell cultures and clarified cell culture supernatant, were
visualized under transmission electron microscopy .Virus titration utilizing BB cells for the
Birnavirus - like isolate achieved a mean titre of 7.40 X 105
TC ID50/ml. Relatively high virus titres were also recorded from the other two isolants , with a mean virus titres of
7.16 X 105 TC ID50/ml for Herpesvirus - like and 6.4 6 'x 105
TCID50/ml for Iridovirus-like . |
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