Studies On Malaysian Isolates Of Orf Virus
This study was conducted to isolate and characterize several orf viruses infecting sheep and goats in Malaysia. The isolates and two reference strains of orf virus were then compared with respect to biological properties, serological properties, protein profile and also DNA profile. Three orf vi...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
1995
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| Online Access: | http://psasir.upm.edu.my/id/eprint/12317/1/FPV_1995_5_A.pdf http://psasir.upm.edu.my/id/eprint/12317/ |
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| Institution: | Universiti Putra Malaysia |
| Language: | English English |
| Summary: | This study was conducted to isolate and characterize several orf viruses
infecting sheep and goats in Malaysia. The isolates and two reference strains of
orf virus were then compared with respect to biological properties, serological
properties, protein profile and also DNA profile.
Three orf viruses were isolated from infected goats and one from infected
sheep. They were identified as orf viruses by means of electron microscope and
three serologic tests namely, serum neutralization, immunoperoxidase and
immunofluorescent tests. The isolates grew well in cell cultures prepared from
goat and sheep, and on chorioallantoic membrane of hen eggs but not in several
tested continuous cell lines.
Compared to the other two established orf virus strains, cytopathic effect
induced by these isolates in kid testis cell culture differed with respect to the time |
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