Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid

Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid

To construct an expression vector for Lactococcus lactis, the EmPMT fragment which contained the erythromycin resistance gene, P32 promoter, multiple cloning site (MCS) and terminator (T) was subcloned into the small cryptic plasmid pAR141. The resulting vector, designated as pAR1411, was found to b...

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Main Authors: Hooi, Wei Yeng, Shamsudin, Mariana Nor, Abdul Rahim, Raha
Format: Article
Language:English
Published: Academic Journals 2009
Online Access:http://psasir.upm.edu.my/id/eprint/13757/1/13757.pdf
http://psasir.upm.edu.my/id/eprint/13757/
http://www.academicjournals.org/journal/AJB/article-abstract/84F18C88334
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Institution: Universiti Putra Malaysia
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spelling my.upm.eprints.137572017-11-13T02:37:56Z http://psasir.upm.edu.my/id/eprint/13757/ Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid Hooi, Wei Yeng Shamsudin, Mariana Nor Abdul Rahim, Raha To construct an expression vector for Lactococcus lactis, the EmPMT fragment which contained the erythromycin resistance gene, P32 promoter, multiple cloning site (MCS) and terminator (T) was subcloned into the small cryptic plasmid pAR141. The resulting vector, designated as pAR1411, was found to be stably maintained in L. lactis MG1363 after transformation for at least 100 generations under non-selective conditions. The vector was also demonstrated to be able to express the gene coding for chloramphenicol acetyltransferase (cat) in L. lactis. Academic Journals 2009-11-02 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13757/1/13757.pdf Hooi, Wei Yeng and Shamsudin, Mariana Nor and Abdul Rahim, Raha (2009) Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid. African Journal of Biotechnology, 8 (21). art. no. 84F18C88334. pp. 5621-5626. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/84F18C88334
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description To construct an expression vector for Lactococcus lactis, the EmPMT fragment which contained the erythromycin resistance gene, P32 promoter, multiple cloning site (MCS) and terminator (T) was subcloned into the small cryptic plasmid pAR141. The resulting vector, designated as pAR1411, was found to be stably maintained in L. lactis MG1363 after transformation for at least 100 generations under non-selective conditions. The vector was also demonstrated to be able to express the gene coding for chloramphenicol acetyltransferase (cat) in L. lactis.
format Article
author Hooi, Wei Yeng
Shamsudin, Mariana Nor
Abdul Rahim, Raha
spellingShingle Hooi, Wei Yeng
Shamsudin, Mariana Nor
Abdul Rahim, Raha
Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid
author_facet Hooi, Wei Yeng
Shamsudin, Mariana Nor
Abdul Rahim, Raha
author_sort Hooi, Wei Yeng
title Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid
title_short Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid
title_full Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid
title_fullStr Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid
title_full_unstemmed Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid
title_sort construction of an expression vector for lactococcus lactis based on an indigenous cryptic plasmid
publisher Academic Journals
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/13757/1/13757.pdf
http://psasir.upm.edu.my/id/eprint/13757/
http://www.academicjournals.org/journal/AJB/article-abstract/84F18C88334
_version_ 1643825426929287168

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