Controlled expression of cholera toxin B subunit from Vibro cholerae in Escherichia coli.

• Main Authors: Toosa, Haryanti, Shamsudin , Mariana Nor, Saiful Yazan, Latifah, Yusoff, Khatijah, Abdul Rahim, Raha
• Format: Article
• Language: English; English
• Published: Asian Network for Scientific Information 2008
• Online Access: http://psasir.upm.edu.my/id/eprint/13785/1/Controlled%20expression%20of%20cholera%20toxin%20B%20subunit%20from%20Vibro%20cholerae%20in%20Escherichia%20coli.pdf; http://psasir.upm.edu.my/id/eprint/13785/
• Institution: Universiti Putra Malaysia

The ctxB gene, the causative agent of cholera epidemic was successfully cloned from V. cholerae in E. coli. The insertion of the gene was confirmed by PCR as well as restriction digestion analyses. The sequencing results for the gene confirmed that the insert was in the correct orientation and in-fr...