Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems.

• Main Authors: Ooi, Chien Wei, Tey, Beng Ti, Hii, Siew Ling, Ariff, Arbakariya, Wu, Ho Shing, Lan, John Chi Wei, Juang, RueyShin, Mustapa Kamal, Siti Mazlina, Chuan, Ling Tau
• Format: Article
• Language: English
• Published: Springer 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/14082/
• Institution: Universiti Putra Malaysia

An aqueous two-phase purification process was employed for the recovery of Burkholderia Pseudomallei lipase from fermentation broth. The partition behavior of Burkholderia Pseudomallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR...