Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor
Shrimps have been a popular raw material for the burgeoning marine and food industry contributing to increasing marine waste. Shrimp waste, which is rich in organic compounds is an abundant source of chitin, a natural polymer of N-acetyl-D-glucosamine (GluNac), a reducing sugar. For this respect, ch...
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Humana Press
2008
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Online Access: | http://psasir.upm.edu.my/id/eprint/14203/1/Effect%20of%20agitation%20and%20aeration%20rates%20on%20chitinase%20production%20using%20Trichoderma%20virens%20UKM1%20in%202%20L%20stirred%20tank%20reactor.pdf http://psasir.upm.edu.my/id/eprint/14203/ |
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my.upm.eprints.142032015-12-03T03:54:57Z http://psasir.upm.edu.my/id/eprint/14203/ Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor Abd. Aziz, Suraini Hassan, Mohd Ali Fernandez, Christine C. Md Salleh, Madihah Md Illias, Rosli Shrimps have been a popular raw material for the burgeoning marine and food industry contributing to increasing marine waste. Shrimp waste, which is rich in organic compounds is an abundant source of chitin, a natural polymer of N-acetyl-D-glucosamine (GluNac), a reducing sugar. For this respect, chitinase-producing fungi have been extensively studied as biocontrol agents. Locally isolated Trichoderma virens UKM1 was used in this study. The effect of agitation and aeration rates using colloidal chitin as control substrate in a 2-l stirred tank reactor gave the best agitation and aeration rates at 200 rpm and 0.33 vvm with 4.1 U/l per hour and 5.97 U/l per hour of maximum volumetric chitinase activity obtained, respectively. Microscopic observations showed shear sensitivity at higher agitation rate of the above system. The oxygen uptake rate during the highest chitinase productivity obtained using sun-dried ground shrimp waste of 1.74 mg of dissolved oxygen per gram of fungal biomass per hour at the kappaL a of 8.34 per hour. Humana Press 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14203/1/Effect%20of%20agitation%20and%20aeration%20rates%20on%20chitinase%20production%20using%20Trichoderma%20virens%20UKM1%20in%202%20L%20stirred%20tank%20reactor.pdf Abd. Aziz, Suraini and Hassan, Mohd Ali and Fernandez, Christine C. and Md Salleh, Madihah and Md Illias, Rosli (2008) Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor. Applied Biochemistry and Biotechnology, 150 (2). pp. 193-204. ISSN 0273-2289 English |
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Shrimps have been a popular raw material for the burgeoning marine and food industry contributing to increasing marine waste. Shrimp waste, which is rich in organic compounds is an abundant source of chitin, a natural polymer of N-acetyl-D-glucosamine (GluNac), a reducing sugar. For this respect, chitinase-producing fungi have been extensively studied as biocontrol agents. Locally isolated Trichoderma virens UKM1 was used in this study. The effect of agitation and aeration rates using colloidal chitin as control substrate in a 2-l stirred tank reactor gave the best agitation and aeration rates at 200 rpm and 0.33 vvm with 4.1 U/l per hour and 5.97 U/l per hour of maximum volumetric chitinase activity obtained, respectively. Microscopic observations showed shear sensitivity at higher agitation rate of the above system. The oxygen uptake rate during the highest chitinase productivity obtained using sun-dried ground shrimp waste of 1.74 mg of dissolved oxygen per gram of fungal biomass per hour at the kappaL a of 8.34 per hour. |
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Abd. Aziz, Suraini Hassan, Mohd Ali Fernandez, Christine C. Md Salleh, Madihah Md Illias, Rosli |
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Abd. Aziz, Suraini Hassan, Mohd Ali Fernandez, Christine C. Md Salleh, Madihah Md Illias, Rosli Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor |
author_facet |
Abd. Aziz, Suraini Hassan, Mohd Ali Fernandez, Christine C. Md Salleh, Madihah Md Illias, Rosli |
author_sort |
Abd. Aziz, Suraini |
title |
Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor |
title_short |
Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor |
title_full |
Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor |
title_fullStr |
Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor |
title_full_unstemmed |
Effect of agitation and aeration rates on chitinase production using Trichoderma virens UKM1 in 2 L stirred tank reactor |
title_sort |
effect of agitation and aeration rates on chitinase production using trichoderma virens ukm1 in 2 l stirred tank reactor |
publisher |
Humana Press |
publishDate |
2008 |
url |
http://psasir.upm.edu.my/id/eprint/14203/1/Effect%20of%20agitation%20and%20aeration%20rates%20on%20chitinase%20production%20using%20Trichoderma%20virens%20UKM1%20in%202%20L%20stirred%20tank%20reactor.pdf http://psasir.upm.edu.my/id/eprint/14203/ |
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