Effect of different operating modes and biomass concentrations on the recovery of recombinant hepatitis B core antigen from thermal-treated unclarified Escherichia coli feedstock

• Main Authors: Ng, Michelle Yeen Tan, Tan, Wen Siang, Abdullah, Norhafizah, Ling, Tau Chuan, Tey , Beng Ti
• Format: Article
• Language: English; English
• Published: Elsevier B.V. 2008
• Online Access: http://psasir.upm.edu.my/id/eprint/14228/1/Effect%20of%20different%20operating%20modes%20and%20biomass%20concentrations%20on%20the%20recovery%20of%20recombinant%20hepatitis%20B%20core%20antigen%20from%20thermal.pdf; http://psasir.upm.edu.my/id/eprint/14228/
• Institution: Universiti Putra Malaysia

Expanded bed adsorption chromatography (EBAC) is a single pass operation that has been used as primary capture step in various protein purifications. The most common problem in EBAC is often associated with successful formation of a stable fluidized bed during the absorption stage, which is critical...