Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent

• Main Authors: Ling, Tau Chuan, Lyddiatt, Andrew
• Format: Article
• Language: English
• Published: The Korean Society for Biotechnology and Bioengineering 2006
• Online Access: http://psasir.upm.edu.my/id/eprint/15491/1/Effect%20of%20polymer%20shielding%20on%20elution%20of%20G3PDH%20bound%20to%20dye-ligand%20adsorbent.pdf; http://psasir.upm.edu.my/id/eprint/15491/; https://link.springer.com/article/10.1007/BF02931874
• Institution: Universiti Putra Malaysia

Batch binding experiments were performed to assess the recovery performance of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) bound to the unshielded and polymer (polyvinyl pyrrolidone, PVP)-shielded dye-ligand (Cibacron Blue 3GA) adsorbent. The adoption of a polymer-shielded, dye-ligand technique facilitated the elution efficiency of bound G3PDH. It was demonstrated that the recovery of G3PDH using polymer-shielded dye-ligand adsorption yielded higher elution efficiency, at 60.5% and a specific activity of 42.3 IU/mg, after a low ionic strength elution (0.15 M NaCl). The unshielded dye-ligand yielded lower elution efficiency, at 6.5% and a specific activity of 10.2 IU/mg.