Molecular investigation of a gene encoding organic solvent-tolerant alkaline protease from Pseudomonas aeruginosa strain K

• Main Authors: Raja Abdul Rahman, Raja Noor Zaliha, Lee, Poh Geok, Wong, Chee Fah, Basri, Mahiran, Salleh, Abu Bakar
• Format: Article
• Language: English
• Published: Wiley-VCH Verlag GmbH & Co. KGaA 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/15709/1/15709.pdf; http://psasir.upm.edu.my/id/eprint/15709/; http://onlinelibrary.wiley.com/wol1/doi/10.1002/jobm.200900133/abstract
• Institution: Universiti Putra Malaysia

A gene encoding an organic solvent-stable protease was amplified from Pseudomonas aeruginosa strain K by polymerase chain reaction using consensus primers based on multiple sequence alignment of alkaline and metalloprotease genes from Pseudomonas species. The gene, which consisted of 1440 bp nucleot...