Primary capture of high molecular weight nucleic acids using aqueous two-phase systems

Primary capture of high molecular weight nucleic acids using aqueous two-phase systems

The practical feasibility and generic applicability of the selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol 300 (PEG-300) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) by addition of NaCl salt was investigated with partially disrupted Escherichia coli (...

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Main Authors: Luechau, Frank, Ling, Tau Chuan, Lyddiatt, Andrew
Format: Article
Language:English
Published: Elsevier 2009
Online Access:http://psasir.upm.edu.my/id/eprint/16338/1/Primary%20capture%20of%20high%20molecular%20weight%20nucleic%20acids%20using%20aqueous%20two.pdf
http://psasir.upm.edu.my/id/eprint/16338/
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Institution: Universiti Putra Malaysia
Language: English
id my.upm.eprints.16338
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spelling my.upm.eprints.163382015-10-05T05:53:36Z http://psasir.upm.edu.my/id/eprint/16338/ Primary capture of high molecular weight nucleic acids using aqueous two-phase systems Luechau, Frank Ling, Tau Chuan Lyddiatt, Andrew The practical feasibility and generic applicability of the selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol 300 (PEG-300) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) by addition of NaCl salt was investigated with partially disrupted Escherichia coli (E. coli) cell paste. The process yielded a relatively protein-free pDNA solution with 89.5% pDNA recovery while low molecular weight RNA was partially removed. The results gained here led the way to an alternative process options for the processing of pDNA. A reproducible process involving two extraction steps to recover pDNA from partially disrupted E. coli cell paste was developed and is presented here. Elsevier 2009-04-07 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16338/1/Primary%20capture%20of%20high%20molecular%20weight%20nucleic%20acids%20using%20aqueous%20two.pdf Luechau, Frank and Ling, Tau Chuan and Lyddiatt, Andrew (2009) Primary capture of high molecular weight nucleic acids using aqueous two-phase systems. Separation and Purification Technology, 66 (1). pp. 202-207. ISSN 1383-5866; ESSN: 1873-3794 10.1016/j.seppur.2008.12.008
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The practical feasibility and generic applicability of the selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol 300 (PEG-300) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) by addition of NaCl salt was investigated with partially disrupted Escherichia coli (E. coli) cell paste. The process yielded a relatively protein-free pDNA solution with 89.5% pDNA recovery while low molecular weight RNA was partially removed. The results gained here led the way to an alternative process options for the processing of pDNA. A reproducible process involving two extraction steps to recover pDNA from partially disrupted E. coli cell paste was developed and is presented here.
format Article
author Luechau, Frank
Ling, Tau Chuan
Lyddiatt, Andrew
spellingShingle Luechau, Frank
Ling, Tau Chuan
Lyddiatt, Andrew
Primary capture of high molecular weight nucleic acids using aqueous two-phase systems
author_facet Luechau, Frank
Ling, Tau Chuan
Lyddiatt, Andrew
author_sort Luechau, Frank
title Primary capture of high molecular weight nucleic acids using aqueous two-phase systems
title_short Primary capture of high molecular weight nucleic acids using aqueous two-phase systems
title_full Primary capture of high molecular weight nucleic acids using aqueous two-phase systems
title_fullStr Primary capture of high molecular weight nucleic acids using aqueous two-phase systems
title_full_unstemmed Primary capture of high molecular weight nucleic acids using aqueous two-phase systems
title_sort primary capture of high molecular weight nucleic acids using aqueous two-phase systems
publisher Elsevier
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/16338/1/Primary%20capture%20of%20high%20molecular%20weight%20nucleic%20acids%20using%20aqueous%20two.pdf
http://psasir.upm.edu.my/id/eprint/16338/
_version_ 1643826184520204288

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