A tapered fibre optics biosensor for histamine detection

• Main Authors: Usman, Hamza, Abu Bakar, Muhammad Hafiz, Amir Hamzah, Amir Syahir, Salleh, Abu Bakar
• Format: Article
• Language: English
• Published: Emerald Group Publishing 2016
• Online Access: http://psasir.upm.edu.my/id/eprint/16876/1/A%20tapered%20fibre%20optics%20biosensor%20for%20histamine%20detection.pdf; http://psasir.upm.edu.my/id/eprint/16876/; http://www.emeraldinsight.com/doi/abs/10.1108/SR-06-2015-0101
• Institution: Universiti Putra Malaysia

Purpose: This paper aims to estimate the level of histamine in fish and fish products, as it is very important because of their implication in fish poisoning in humans; hence, ascertaining histamine levels in the aforementioned serves as a chemical index for spoilage. Design/methodology/approach: A technique was developed to immobilize an ordered multilayer of diamine oxidase (DAO) by means of chemical cross-linking on the biconical taper surface stepwisely alternating between chitosan, glutaraldehyde and the enzyme. A spectrophotometric signal results from horseradish peroxidase catalyzed reduction of H2O2, a secondary product of the oxidative deamination of histamine monitored at 450 nm. Findings: The biosensor showed a linear response range up to 1.5 mM, a good sensitivity of 0.64 mM-1 with detection and quantification limits towards histamine of 0.086 mM (15.8 ppm) and 0.204 mM (37.7 ppm) and a linear response range of 0-1.5 mM. It showed a response and recovery time of 14 sec and operational stability up to 40 repeated analyses without significant loss of sensitivity. Practical implications: The developed biosensor has a good potential for use in the quantitative determination of histamine in seafood. Originality/value: The paper described an outcome of an experimental work on tapered fibre optics (taper)-based biosensor coated with DAO embedded into a chitosan membrane to measure histamine.