Using RAPD-PCR as molecular assessment on the performance of CHROMAgar™ Listeria and PALCAM agar on isolation of Listeria spp. and L. monocytogenes from foods
In this study, Listeria spp. were isolated from naturally contaminated samples of beef burger, minced beef and sliced cheese using PALCAM and CHROMagar™. Samples were enriched with FDA-BAM method and plated on PALCAM and CHROMagar™ Listeria before confirmation using PCR on hlyA and LLO toxin genes...
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2011
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my.upm.eprints.222362015-06-02T03:59:45Z http://psasir.upm.edu.my/id/eprint/22236/ Using RAPD-PCR as molecular assessment on the performance of CHROMAgar™ Listeria and PALCAM agar on isolation of Listeria spp. and L. monocytogenes from foods Lee, Hai Yen Chai, Lay Ching Pui, Chai Fung Robin, Tunung Wong, Woan Chwen Mustafa, Shuhaimi Cheah, Yoke Kqueen Mohamad Ghazali, Farinazleen Nishibuchi, Mitsuaki Radu, Son In this study, Listeria spp. were isolated from naturally contaminated samples of beef burger, minced beef and sliced cheese using PALCAM and CHROMagar™. Samples were enriched with FDA-BAM method and plated on PALCAM and CHROMagar™ Listeria before confirmation using PCR on hlyA and LLO toxin genes specific to L. monocytogenes. Identification of isolates showed a total of 45 isolates of Listeria spp. and two L. monocytogenes. All the 47 isolates were then subjected to RAPD analysis using two oligomers (OPA14 and OPA15) and fingerprint clustering was able to cluster the L. monocytogenes from Listeria spp. based on isolation from agar types as well as L. monocytogenes from Listeria spp. Studies showed that OPA14 and OPA15 are useful for rapid discrimination of Listeria spp. and L. monocytogenes . The differences observed on the isolation of Listeria spp. from PALCAM and CHROMagar™ Listeria that may have an impact on epidemiological studies. Faculty of Food Science and Technology, Universiti Putra Malaysia 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22236/1/22236.pdf Lee, Hai Yen and Chai, Lay Ching and Pui, Chai Fung and Robin, Tunung and Wong, Woan Chwen and Mustafa, Shuhaimi and Cheah, Yoke Kqueen and Mohamad Ghazali, Farinazleen and Nishibuchi, Mitsuaki and Radu, Son (2011) Using RAPD-PCR as molecular assessment on the performance of CHROMAgar™ Listeria and PALCAM agar on isolation of Listeria spp. and L. monocytogenes from foods. International Food Research Journal, 18 (2). pp. 501-506. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/volume-18-2011.html English |
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In this study, Listeria spp. were isolated from naturally contaminated samples of beef burger, minced beef and sliced cheese using PALCAM and CHROMagar™. Samples were enriched with FDA-BAM method and plated on PALCAM and CHROMagar™
Listeria before confirmation using PCR on hlyA and LLO toxin
genes specific to L. monocytogenes. Identification of isolates showed a total of 45 isolates of Listeria
spp. and two L. monocytogenes. All the 47 isolates were then subjected to RAPD analysis using two oligomers (OPA14
and OPA15) and fingerprint clustering was able to cluster the L. monocytogenes from Listeria spp. based on isolation from agar types as well as L. monocytogenes from Listeria
spp. Studies showed that OPA14 and OPA15 are useful for rapid discrimination of Listeria spp. and L. monocytogenes
. The differences observed on the isolation of Listeria
spp. from PALCAM and CHROMagar™ Listeria that may have an impact on epidemiological studies. |
format |
Article |
author |
Lee, Hai Yen Chai, Lay Ching Pui, Chai Fung Robin, Tunung Wong, Woan Chwen Mustafa, Shuhaimi Cheah, Yoke Kqueen Mohamad Ghazali, Farinazleen Nishibuchi, Mitsuaki Radu, Son |
spellingShingle |
Lee, Hai Yen Chai, Lay Ching Pui, Chai Fung Robin, Tunung Wong, Woan Chwen Mustafa, Shuhaimi Cheah, Yoke Kqueen Mohamad Ghazali, Farinazleen Nishibuchi, Mitsuaki Radu, Son Using RAPD-PCR as molecular assessment on the performance of CHROMAgar™ Listeria and PALCAM agar on isolation of Listeria spp. and L. monocytogenes from foods |
author_facet |
Lee, Hai Yen Chai, Lay Ching Pui, Chai Fung Robin, Tunung Wong, Woan Chwen Mustafa, Shuhaimi Cheah, Yoke Kqueen Mohamad Ghazali, Farinazleen Nishibuchi, Mitsuaki Radu, Son |
author_sort |
Lee, Hai Yen |
title |
Using RAPD-PCR as molecular assessment on the performance of CHROMAgar™ Listeria and PALCAM agar on isolation of Listeria spp. and L. monocytogenes from foods |
title_short |
Using RAPD-PCR as molecular assessment on the performance of CHROMAgar™ Listeria and PALCAM agar on isolation of Listeria spp. and L. monocytogenes from foods |
title_full |
Using RAPD-PCR as molecular assessment on the performance of CHROMAgar™ Listeria and PALCAM agar on isolation of Listeria spp. and L. monocytogenes from foods |
title_fullStr |
Using RAPD-PCR as molecular assessment on the performance of CHROMAgar™ Listeria and PALCAM agar on isolation of Listeria spp. and L. monocytogenes from foods |
title_full_unstemmed |
Using RAPD-PCR as molecular assessment on the performance of CHROMAgar™ Listeria and PALCAM agar on isolation of Listeria spp. and L. monocytogenes from foods |
title_sort |
using rapd-pcr as molecular assessment on the performance of chromagar™ listeria and palcam agar on isolation of listeria spp. and l. monocytogenes from foods |
publisher |
Faculty of Food Science and Technology, Universiti Putra Malaysia |
publishDate |
2011 |
url |
http://psasir.upm.edu.my/id/eprint/22236/1/22236.pdf http://psasir.upm.edu.my/id/eprint/22236/ http://www.ifrj.upm.edu.my/volume-18-2011.html |
_version_ |
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