Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii.

Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii.

Seven single locus microsatellite markers were characterized in Malaysian giant freshwater prawn, Macrobrachium rosenbergii from an enriched genomic library Primer pairs were designed to flank the repeat sequences and the loci characterized for this species. The bands resulting from the PCR amplific...

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Main Authors: See, Leng Min, Hassan, Rosly, Tan, Soon Guan, Bhassu, Subha
Format: Article
Language:English
Published: Springer 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22272/
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Institution: Universiti Putra Malaysia
Language: English
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spelling my.upm.eprints.222722014-10-13T07:10:50Z http://psasir.upm.edu.my/id/eprint/22272/ Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii. See, Leng Min Hassan, Rosly Tan, Soon Guan Bhassu, Subha Seven single locus microsatellite markers were characterized in Malaysian giant freshwater prawn, Macrobrachium rosenbergii from an enriched genomic library Primer pairs were designed to flank the repeat sequences and the loci characterized for this species. The bands resulting from the PCR amplifications of these eight microsatellite loci were polymorphic with the number of alleles ranging from 8 to 26 alleles per locus, whereas the observed heterozygosity ranged from 0.0641 to 0.6564. These newly developed microsatellite markers should prove to be useful for population studies and in the management of genetic variations in broodstocks of freshwater prawn, M. rosenbergii. Springer 2011 Article PeerReviewed See, Leng Min and Hassan, Rosly and Tan, Soon Guan and Bhassu, Subha (2011) Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii. Russian Journal of Genetics, 47 (4). pp. 497-499. ISSN 1022-7954 10.1134/S102279541103015X English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Seven single locus microsatellite markers were characterized in Malaysian giant freshwater prawn, Macrobrachium rosenbergii from an enriched genomic library Primer pairs were designed to flank the repeat sequences and the loci characterized for this species. The bands resulting from the PCR amplifications of these eight microsatellite loci were polymorphic with the number of alleles ranging from 8 to 26 alleles per locus, whereas the observed heterozygosity ranged from 0.0641 to 0.6564. These newly developed microsatellite markers should prove to be useful for population studies and in the management of genetic variations in broodstocks of freshwater prawn, M. rosenbergii.
format Article
author See, Leng Min
Hassan, Rosly
Tan, Soon Guan
Bhassu, Subha
spellingShingle See, Leng Min
Hassan, Rosly
Tan, Soon Guan
Bhassu, Subha
Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii.
author_facet See, Leng Min
Hassan, Rosly
Tan, Soon Guan
Bhassu, Subha
author_sort See, Leng Min
title Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii.
title_short Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii.
title_full Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii.
title_fullStr Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii.
title_full_unstemmed Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii.
title_sort novel polymorphic microsatellite dna markers from malaysian giant freshwater prawn, macrobrachium rosenbergii.
publisher Springer
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/22272/
_version_ 1643827779579412480

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