An effective extracellular protein secretion by an ABC transporter system in Escherichia coli: statistical modeling and optimization of cyclodextrin glucanotransferase secretory production

• Main Authors: Kheng, Oon Low, Mahadi, Nor Muhammad, Abdul Rahim, Raha, Rabu, Amir, Abu Bakar, Farah Diba, Abdul Murad, Abdul Munir, Md. Illias, Rosli
• Format: Article
• Language: English
• Published: Springer-Verlag 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/22354/1/An%20effective%20extracellular%20protein%20secretion%20by%20an%20ABC%20transporter%20system%20in%20Escherichia%20coli.pdf; http://psasir.upm.edu.my/id/eprint/22354/; http://link.springer.com/article/10.1007%2Fs10295-011-0949-0
• Institution: Universiti Putra Malaysia

Direct transport of recombinant protein from cytosol to extracellular medium offers great advantages, such as high specific activity and a simple purification step. This work presents an investigation on the potential of an ABC (ATP-binding cassette) transporter system, the hemolysin transport syste...