Preliminary analysis of cryopreservation of Dendrobium Bobby Messina orchid using an encapsulation-dehydration technique with Evans blue assay

• Main Authors: Zainuddin, Masitah, Julkifle, Advina Lizah, Pobathy, Ranjetta, Sinniah, Uma Rani, Khoddamzadeh, Amir, James Antony, Jessica Jeyanthi, Pavallekoodi, Subramaniam, Sreeramanan
• Format: Article
• Language: English
• Published: Academic Journals 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/23681/1/23681.pdf; http://psasir.upm.edu.my/id/eprint/23681/; http://www.academicjournals.org/journal/AJB/article-abstract/75981B137092
• Institution: Universiti Putra Malaysia

In vitro grown protocorm-like bodies (PLBs) of Dendrobium Bobby Messina hybrid were cryopreserved in liquid nitrogen (LN) at -196°C by an encapsulation-dehydration technique. PLBs (1 to 2 and 3 to 4 mm) were precultured in half strength semi-solid MS media supplemented with six different concentrati...