Molecular cloning and extracellular expression of cyclodextrin glycosyltransferase gene from Bacillus sp. NR5 UPM

• Main Authors: Ramli, Norhayati, Abd. Aziz, Suraini, Hassan, Mohd Ali, Mohammed Alitheen, Noorjahan Banu, Kamaruddin, Kamarulzaman, Ibrahim, Zoolhilmi
• Format: Article
• Language: English
• Published: Academic Journals 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/24091/1/24091.pdf; http://psasir.upm.edu.my/id/eprint/24091/; http://www.academicjournals.org/journal/AJMR/article-abstract/3BF03B713165
• Institution: Universiti Putra Malaysia

The cloning of a polymerase chain reaction (PCR) gene fragment from Bacillus sp. NR5 UPM isolated from the soil in Malaysia into an Escherichia coli expression vector was successfully carried out. Analysis of the nucleotide sequences revealed the presence of an open reading frame of 2112 bp which en...