Three new amides from Streptomyces sp. H7372

• Main Authors: Cheenpracha, Sarot, Borris, Robert P., Tran, Tammy T., Jee, Jap Meng, Seow, Heng Fong, Cheah, Hwen Yee, Ho, Coy Choke, Chang, Leng Chee
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/24724/1/Three%20new%20amides%20from%20streptomyces%20sp.pdf; http://psasir.upm.edu.my/id/eprint/24724/
• Institution: Universiti Putra Malaysia

Three new amides, methyl phenatate A (1), actiphenamide (2) and actiphenol 1-β-D- glucopyranoside (3), along with thirteen known compounds, were isolated from the organic extract of a fermentation culture of Streptomyces sp. H7372. The structures were elucidated by spectroscopic methods including 1D- and 2D-NMR techniques, and MS analyses. Cycloheximide (6) and cyclo(δAla-L- Val) (8) gave a clear zone of inhibition of Ras-Raf-1 interaction in the yeast two- hybrid assay which showed high potency with 10 and 25 mm clear ZOIs on SD His - and inactive on SD His + at 2.5 μg per disk, respectively.