Antinociceptive, anti-inflammatory and antipyretic effects of Solanum nigrum chloroform extract in animal models

• Main Authors: Zakaria, Zainul Amiruddin, Gopalan, Hanan Kumar, Zainal, Hairani, Mohd Pojan, Nur Hidayu, Morsid, Nur Atiqah, Aris, Anwariah, Sulaiman, Mohd Roslan
• Format: Article
• Language: English
• Published: The Pharmaceutical Society of Japan 2006
• Online Access: http://psasir.upm.edu.my/id/eprint/25549/1/Antinociceptive%2C%20anti-inflammatory%20and%20antipyretic%20effects%20of%20Solanum%20nigrum%20chloroform%20extract%20in%20animal%20models.pdf; http://psasir.upm.edu.my/id/eprint/25549/; https://www.jstage.jst.go.jp/article/yakushi/126/11/126_11_1171/_article
• Institution: Universiti Putra Malaysia

AIM: The present study was carried out to evaluate the antinociceptive, anti-inflammatory and antipyretic effects of chloroform extract of Solanum nigrum leaves using various animal models. METHODS: The extract was prepared by soaking (1:20; w/v) the air-dried powdered leaves (20 g) in chloroform for 72 hrs followed by evaporation (40°C) under reduced pressure to dryness (1.26 g) and then dissolved (1:50; w/v) in dimethylsulfoxide (DMSO). The supernatant, considered as the stock solution with dose of 200 mg/kg, was diluted using DMSO to 20 and 100 mg/kg, and all doses were administered (s.c.; 10 ml/kg) in mice/rats 30 min prior to tests. RESULTS: The extract exhibited significant (p<0.05) antinociceptive activity when assessed using the abdominal constriction, hot plate and formalin tests. The extract also produced significant (p<0.05) anti-inflammatory and antipyretic activities when assessed using the carrageenan-induced paw edema and brewer's yeast-induced pyrexia tests. Overall, the activities occurred in a dose-independent manner. CONCLUSION: The present study demonstrated that the lipid-soluble extract of S. nigrum leaves possessed antinociceptive, anti-inflammatory and anti-pyretic properties and confirmed the traditional claims.