Improvement of thermal stability via outer-loop ion pair interaction of mutated T1 lipase from Geobacillus zalihae strain T1

Improvement of thermal stability via outer-loop ion pair interaction of mutated T1 lipase from Geobacillus zalihae strain T1

Mutant D311E and K344R were constructed using site-directed mutagenesis to introduce an additional ion pair at the inter-loop and the intra-loop, respectively, to determine the effect of ion pairs on the stability of T1 lipase isolated from Geobacillus zalihae. A series of purification steps was app...

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Bibliographic Details
Main Authors: Ruslan, Rudzanna, Raja Abdul Rahman, Raja Noor Zaliha, Leow, Thean Chor, Mohamad Ali, Mohd Shukuri, Basri, Mahiran, Salleh, Abu Bakar
Format: Article
Language:English
Published: MDPI AG 2012
Online Access:http://psasir.upm.edu.my/id/eprint/27295/1/27295.pdf
http://psasir.upm.edu.my/id/eprint/27295/
http://www.mdpi.com/1422-0067/13/1/943
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Institution: Universiti Putra Malaysia
Language: English

Internet

http://psasir.upm.edu.my/id/eprint/27295/1/27295.pdf
http://psasir.upm.edu.my/id/eprint/27295/
http://www.mdpi.com/1422-0067/13/1/943

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