Acetone–butanol–ethanol production by Clostridium acetobutylicum ATCC 824 using sago pith residues hydrolysate
Sago pith residues (58 % starch, 23 % cellulose, 9.2 % hemicellulose, and 4 % lignin) are one of the abundant lignocellulosic residues generated after starch extraction process in sago mill. In this study, fermentable sugars from enzymatic hydrolysis of sago pith residues were converted to acetone–b...
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my.upm.eprints.280142016-04-21T09:40:39Z http://psasir.upm.edu.my/id/eprint/28014/ Acetone–butanol–ethanol production by Clostridium acetobutylicum ATCC 824 using sago pith residues hydrolysate Linggang, Siren Phang, Lai Yee Wasoh @ Mohamad Isa, Helmi Abd. Aziz, Suraini Sago pith residues (58 % starch, 23 % cellulose, 9.2 % hemicellulose, and 4 % lignin) are one of the abundant lignocellulosic residues generated after starch extraction process in sago mill. In this study, fermentable sugars from enzymatic hydrolysis of sago pith residues were converted to acetone–butanol–ethanol (ABE) by Clostridium acetobutylicum ATCC 824. With an initial concentration of 30 g/L of concentrated sago pith residues hydrolysate containing 23 g/L of glucose and 4.58 g/L of cellobiose, 4.22 ± 0.17 g/L of ABE were produced after 72 h of fermentation with yield and productivity of 0.20 g/g glucose and 0.06 g/L/h, respectively. Results are in agreement when synthetic glucose was used as a carbon source. Increasing sago pith residue hydrolysate to 50 g/L (containing 40 g/L glucose) and supplementing with 0.5 g/L yeast extract, approximately 8.84 ± 0.20 g/L of ABE (5.41 ± 0.10 g/L of butanol) were produced with productivity and yield of 0.12 g/L/h and 0.30 g/g glucose respectively, providing a 52 % improvement. Springer 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28014/1/28014.pdf Linggang, Siren and Phang, Lai Yee and Wasoh @ Mohamad Isa, Helmi and Abd. Aziz, Suraini (2013) Acetone–butanol–ethanol production by Clostridium acetobutylicum ATCC 824 using sago pith residues hydrolysate. BioEnergy Research, 6 (1). pp. 321-328. ISSN 1939-1234; ESSN: 1939-1242 http://link.springer.com/article/10.1007%2Fs12155-012-9260-9 10.1007/s12155-012-9260-9 |
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Sago pith residues (58 % starch, 23 % cellulose, 9.2 % hemicellulose, and 4 % lignin) are one of the abundant lignocellulosic residues generated after starch extraction process in sago mill. In this study, fermentable sugars from enzymatic hydrolysis of sago pith residues were converted to acetone–butanol–ethanol (ABE) by Clostridium acetobutylicum ATCC 824. With an initial concentration of 30 g/L of concentrated sago pith residues hydrolysate containing 23 g/L of glucose and 4.58 g/L of cellobiose, 4.22 ± 0.17 g/L of ABE were produced after 72 h of fermentation with yield and productivity of 0.20 g/g glucose and 0.06 g/L/h, respectively. Results are in agreement when synthetic glucose was used as a carbon source. Increasing sago pith residue hydrolysate to 50 g/L (containing 40 g/L glucose) and supplementing with 0.5 g/L yeast extract, approximately 8.84 ± 0.20 g/L of ABE (5.41 ± 0.10 g/L of butanol) were produced with productivity and yield of 0.12 g/L/h and 0.30 g/g glucose respectively, providing a 52 % improvement. |
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Linggang, Siren Phang, Lai Yee Wasoh @ Mohamad Isa, Helmi Abd. Aziz, Suraini |
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Linggang, Siren Phang, Lai Yee Wasoh @ Mohamad Isa, Helmi Abd. Aziz, Suraini Acetone–butanol–ethanol production by Clostridium acetobutylicum ATCC 824 using sago pith residues hydrolysate |
author_facet |
Linggang, Siren Phang, Lai Yee Wasoh @ Mohamad Isa, Helmi Abd. Aziz, Suraini |
author_sort |
Linggang, Siren |
title |
Acetone–butanol–ethanol production by Clostridium acetobutylicum ATCC 824 using sago pith residues hydrolysate |
title_short |
Acetone–butanol–ethanol production by Clostridium acetobutylicum ATCC 824 using sago pith residues hydrolysate |
title_full |
Acetone–butanol–ethanol production by Clostridium acetobutylicum ATCC 824 using sago pith residues hydrolysate |
title_fullStr |
Acetone–butanol–ethanol production by Clostridium acetobutylicum ATCC 824 using sago pith residues hydrolysate |
title_full_unstemmed |
Acetone–butanol–ethanol production by Clostridium acetobutylicum ATCC 824 using sago pith residues hydrolysate |
title_sort |
acetone–butanol–ethanol production by clostridium acetobutylicum atcc 824 using sago pith residues hydrolysate |
publisher |
Springer |
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2013 |
url |
http://psasir.upm.edu.my/id/eprint/28014/1/28014.pdf http://psasir.upm.edu.my/id/eprint/28014/ http://link.springer.com/article/10.1007%2Fs12155-012-9260-9 |
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