Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1

Oil palm empty fruit bunch (OPEFB) was pretreated with 2% (v/v) HNO3 and degraded by Aspergillus niger EFB1 crude cellulase. Through 2 Level Factorial Design (2LFD), it was found that OPEFB concentration, temperature, incubation time, concentration of Tween 80 and agitation speed have significant ef...

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Main Authors: K., Noratiqah, Md Salleh, Madihah, B., Siti Aisyah, Mohamad, Shaza Eva, Abd. Aziz, Suraini, Kamaruddin, Kamarulzaman
Format: Article
Language:English
Published: Elsevier 2013
Online Access:http://psasir.upm.edu.my/id/eprint/28032/1/28032.pdf
http://psasir.upm.edu.my/id/eprint/28032/
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Institution: Universiti Putra Malaysia
Language: English
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spelling my.upm.eprints.280322016-03-07T07:47:12Z http://psasir.upm.edu.my/id/eprint/28032/ Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1 K., Noratiqah Md Salleh, Madihah B., Siti Aisyah Mohamad, Shaza Eva Abd. Aziz, Suraini Kamaruddin, Kamarulzaman Oil palm empty fruit bunch (OPEFB) was pretreated with 2% (v/v) HNO3 and degraded by Aspergillus niger EFB1 crude cellulase. Through 2 Level Factorial Design (2LFD), it was found that OPEFB concentration, temperature, incubation time, concentration of Tween 80 and agitation speed have significant effect in reducing sugar production. A standard Response Surface Methodology (RSM) design known as Central Composite Design (CCD) was used to optimize the enzymatic degradation condition of OPEFB in rotary drum bioreactor. Reducing sugar level of 1.183 g/L was obtained with the following optimized degradation conditions: 1.95% (w/v) OPEFB, 0.5% (v/v) Tween 80, 55 °C, 87.5 rpm in the incubation period of 3 days and 16 h. The optimal degradation condition improved reducing sugar production by 1.07 fold compared to that before optimization in shake flasks culture. The optimization strategy of enzymatic degradation of OPEFB inside rotary drum bioreactor led to increase in glucose, xylose, arabinose, galactose and mannose production by 3, 2.5, 1.64, 19.37 and 22.52 fold, respectively. The improvement in reducing sugar and polyoses production were comparable with the reduction in OPEFB cellulose and hemicellulose content by 89.32% and 48.17% respectively after enzymatic degradation in optimized condition. Elsevier 2013-06-15 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28032/1/28032.pdf K., Noratiqah and Md Salleh, Madihah and B., Siti Aisyah and Mohamad, Shaza Eva and Abd. Aziz, Suraini and Kamaruddin, Kamarulzaman (2013) Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1. Biochemical Engineering Journal, 75. pp. 8-20. ISSN 1369-703X; ESSN: 1873-295X 10.1016/j.bej.2013.03.007
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Oil palm empty fruit bunch (OPEFB) was pretreated with 2% (v/v) HNO3 and degraded by Aspergillus niger EFB1 crude cellulase. Through 2 Level Factorial Design (2LFD), it was found that OPEFB concentration, temperature, incubation time, concentration of Tween 80 and agitation speed have significant effect in reducing sugar production. A standard Response Surface Methodology (RSM) design known as Central Composite Design (CCD) was used to optimize the enzymatic degradation condition of OPEFB in rotary drum bioreactor. Reducing sugar level of 1.183 g/L was obtained with the following optimized degradation conditions: 1.95% (w/v) OPEFB, 0.5% (v/v) Tween 80, 55 °C, 87.5 rpm in the incubation period of 3 days and 16 h. The optimal degradation condition improved reducing sugar production by 1.07 fold compared to that before optimization in shake flasks culture. The optimization strategy of enzymatic degradation of OPEFB inside rotary drum bioreactor led to increase in glucose, xylose, arabinose, galactose and mannose production by 3, 2.5, 1.64, 19.37 and 22.52 fold, respectively. The improvement in reducing sugar and polyoses production were comparable with the reduction in OPEFB cellulose and hemicellulose content by 89.32% and 48.17% respectively after enzymatic degradation in optimized condition.
format Article
author K., Noratiqah
Md Salleh, Madihah
B., Siti Aisyah
Mohamad, Shaza Eva
Abd. Aziz, Suraini
Kamaruddin, Kamarulzaman
spellingShingle K., Noratiqah
Md Salleh, Madihah
B., Siti Aisyah
Mohamad, Shaza Eva
Abd. Aziz, Suraini
Kamaruddin, Kamarulzaman
Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1
author_facet K., Noratiqah
Md Salleh, Madihah
B., Siti Aisyah
Mohamad, Shaza Eva
Abd. Aziz, Suraini
Kamaruddin, Kamarulzaman
author_sort K., Noratiqah
title Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1
title_short Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1
title_full Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1
title_fullStr Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1
title_full_unstemmed Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1
title_sort statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (opefb) in rotary drum bioreactor using crude cellulase produced from aspergillus niger efb1
publisher Elsevier
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/28032/1/28032.pdf
http://psasir.upm.edu.my/id/eprint/28032/
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