In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates

• Main Authors: Suhaili, Zarizal, Johari, Saiful Azmi, Sajili, Mohammad Hailmi, Yahya, Azifah, Zakaria, Zainul Amiruddin, Mohd Desa, Mohd Nasir, Ali, Abdul Manaf
• Format: Article
• Language: English
• Published: Institute of Research and Development, Walailak University 2013
• Online Access: http://psasir.upm.edu.my/id/eprint/28043/1/28043.pdf; http://psasir.upm.edu.my/id/eprint/28043/; http://wjst.wu.ac.th/index.php/wjst/article/view/262
• Institution: Universiti Putra Malaysia

The aims of this study were to validate the primers developed for molecular-based detection and identification of Malaysian clinical isolates of methicilin-resistance Staphylococcus aureus (MRSA) using in-silico Polymerase Chain Reaction (PCR) and real-time PCR SYBR with Green I. Rapid molecular dia...