Enhancement of the yield of long helical structure of recombinant nucleocapsid protein of Newcastle disease virus

• Main Authors: Yap, Chee Fai, Tan, Wen Siang, Sieo, Chin Chin, Tey, Beng Ti
• Format: Article
• Language: English
• Published: Elsevier 2013
• Online Access: http://psasir.upm.edu.my/id/eprint/28070/1/Enhancement%20of%20the%20yield%20of%20long%20helical%20structure%20of%20recombinant%20nucleocapsid%20protein%20of%20Newcastle%20disease%20virus.pdf; http://psasir.upm.edu.my/id/eprint/28070/; http://www.elsevier.com/locate/procbio
• Institution: Universiti Putra Malaysia

A deletion mutant of the nucleocapsid protein (NPΔc375) of Newcastle disease virus self-assembles into a long helical structure when expressed in Escherichia coli. However, the NPΔc375 subjects to proteolytic activity of host cell endogenous proteases during the protein recovery process. Image analy...